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Cell No. : Cell Name
RCB1632 : ΔKu70ΔRad54B-DT40 update : 2024/01/17 |
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| Comment | Ku70 and Rad54B gene double-deleted DT40 (chicken B cells). | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Takeda, Shunichi | ||
| Originator | Yamaguchi-Iwai, Yuko | |||
| Year of deposit | 2000 | |||
| Another name | #201 | |||
| Original cell | DT40 | |||
| Animal | _avian < Birds | |||
| Tissue | B cell | |||
| Classification | mutant | |||
| Recombinant | recombinant | |||
| Exogene | Ku70-his, Ku70-bsr, Rad54-neo, Rad54-hygro | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_6D41 | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Culture method | 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage cell No | 1.5-2 x 10 5 cells/ml |
| Passage ratio | 1 : 10-20 split | |||
| SC frequency | Subculture : every 2 days | |||
| Temperature | 39.5 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | 90% FBS + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| Others | Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day. | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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