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Cell No. : Cell Name
RCB1631 : ΔRad51+hRad51-DT40  update : 2022/04/05
CommentRad51 gene deleted and human Rad51 gene inserted DT40 (chicken B cell line).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takeda, Shunichi
Originator Sonoda, Eiichiro
Year of deposit 2000
Another name #110
Original cell DT40
Animal avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene pUHGhRad51
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_9U92
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10^(5) cells/ml
Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
2465  Sonoda E, Sasaki, M S, Morrison C, Yamaguchi-Iwai Y, Takata M, Takeda S  Sister chromatid exchanges are mediated by homologous recombination in vertebrate cells.  Mol Cell Biol  1999  19:5166-9  PubMed ID: 10373565  
2460  Sonoda E, Sasaki, M S, Buerstedde, J M, Bezzubova O, Shinohara A, Ogawa H, Takata M, Yamaguchi-Iwai Y, Takeda S  Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death.  EMBO J  1998  17:598-608  PubMed ID: 9430650  

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