Cell No. : Cell Name
RCB1631 : ΔRad51+hRad51-DT40
update : 2024/11/29
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| Comment | Rad51 gene deleted and human Rad51 gene inserted DT40 (chicken B cell line). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takeda, Shunichi
|
| Originator |
Sonoda, Eiichiro
|
| Year of deposit |
2000
|
| Another name |
#110
|
| Original cell |
DT40
|
| Animal |
_avian
< Birds
|
| Tissue |
B cell
|
| Classification |
mutant
|
| Recombinant |
recombinant
|
| Exogene |
pUHGhRad51
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_9U92
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
|
Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
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| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
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Culture information
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Passage cell No |
|
1.5-2 x 10 5 cells/ml
|
| Passage ratio |
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1 : 20 split
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| SC frequency |
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Subculture : every 2 days
|
| Temperature |
|
39.5
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
90% FBS + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
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| Others |
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Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
0
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| Reference |
|
2465
Sonoda E, Sasaki, M S, Morrison C, Yamaguchi-Iwai Y, Takata M, Takeda S
Sister chromatid exchanges are mediated by homologous recombination in vertebrate cells.
Mol Cell Biol
1999
19:5166-9
PubMed ID: 10373565
DOI: 10.1128/MCB.19.7.5166
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2460
Sonoda E, Sasaki, M S, Buerstedde, J M, Bezzubova O, Shinohara A, Ogawa H, Takata M, Yamaguchi-Iwai Y, Takeda S
Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death.
EMBO J
1998
17:598-608
PubMed ID: 9430650
DOI: 10.1093/emboj/17.2.598
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