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Cell No. : Cell Name
RCB1628 : ΔRad54-DT40 update : 2025/02/13 |
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| Comment | Rad54 gene deleted DT40 (chicken B cell line). | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Takeda, Shunichi | ||
| Originator | Sonoda, Eiichiro | |||
| Year of deposit | 2000 | |||
| Another name | #33 | |||
| Original cell | DT40 | |||
| Animal | _avian < Birds | |||
| Tissue | B cell | |||
| Classification | mutant | |||
| Recombinant | recombinant | |||
| Exogene | Rad54-hisD, Rad54-neo | |||
| Lifespan | infinite | |||
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_1T34 | |||
| Medium | Medium List | |||
| Culture type | Suspension cells | |||
| Culture method | General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol | |||
| Antibiotics | Free | |||
| Passage method | dilution | |||
| Culture information | Passage cell No | 1.5-2 x 10 5 cells/ml | ||
| Passage ratio | 1 : 10-20 split | |||
| SC frequency | Subculture : every 2 days | |||
| Temperature | 39.5 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | 90% FBS + 10% DMSO | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| DNA barcoding | OK | |||
| Others | Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day. | |||
| Reference information | Reference | 6 | ||
| User's Publication | 1 | |||
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| Reference |
| 17859 Zhao Z, Wu X, He F, Xiang C, Feng X, Bai X, Liu X, Zhao J, Takeda S, Qing Y. Critical roles of Rad54 in tolerance to apigenin-induced Top1-mediated DNA damage Exp Ther Med 2021 21(5):505 PubMed ID: 33791014 DOI: 10.3892/etm.2021.9936 |
| 17845 Sharma V, Collins LB, Chen TH, Herr N, Takeda S, Sun W, Swenberg JA, Nakamura J. Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations Oncotarget 2016 7(18):25377-90 PubMed ID: 27015367 DOI: 10.18632/oncotarget.8298 |
| 3435 Mizutani A, Okada T, Shibutani S, Sonoda E, Hochegger H, Nishigori C, Miyachi Y, Takeda S, Yamazoe M. Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells. Cancer Res. 2004 64(9):3144-7 PubMed ID: 15126352 DOI: 10.1158/0008-5472.can-03-3489 |
| 2465 Sonoda E, Sasaki, M S, Morrison C, Yamaguchi-Iwai Y, Takata M, Takeda S Sister chromatid exchanges are mediated by homologous recombination in vertebrate cells. Mol Cell Biol 1999 19:5166-9 PubMed ID: 10373565 DOI: 10.1128/MCB.19.7.5166 |
| 2464 Takata M, Sasaki, M S, Sonoda E, Morrison C, Hashimoto M, Utsumi H, Yamaguchi-Iwai Y, Shinohara A, Takeda S Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells. EMBO J 1998 17:5497-508 PubMed ID: 9736627 DOI: 10.1093/emboj/17.18.5497 |
| 2463 Bezzubova O, Silbergleit A, Yamaguchi-Iwai Y, Takeda S, Buerstedde, J M Reduced X-ray resistance and homologous recombination frequencies in a RAD54-/- mutant of the chicken DT40 cell line. Cell 1997 89:185-93 PubMed ID: 9108474 DOI: 10.1016/s0092-8674(00)80198-1 |
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| User's Publication |
| 3086 Yin, Hong Lan, Suzuki, Yuka, Matsumoto, Yoshihisa, Tomita, Masanori, Furusawa, Yoshiya, Enomoto, Atsushi, Morita, Akinori, Aoki, Mizuho, Yatagai, Fumio, Suzuki, Takahiko, Hosoi, Yoshio, Ohtomo, Kuni, Suzuki, Norio Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair. Radiat Res 2004 162:433-41 PubMed ID: 15447039 DOI: 10.1667/rr3239 |
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