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Cell No. : Cell Name
RCB1628 : ΔRad54-DT40  update : 2022/04/05
CommentRad54 gene deleted DT40 (chicken B cell line).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takeda, Shunichi
Originator Sonoda, Eiichiro
Year of deposit 2000
Another name #33
Original cell DT40
Animal avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene Rad54-hisD, Rad54-neo
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1T34
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10^(5) cells/ml
Passage ratio 1 : 10-20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information Reference 4
User's Publication 1


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Reference
3435  Mizutani A, Okada T, Shibutani S, Sonoda E, Hochegger H, Nishigori C, Miyachi Y, Takeda S, Yamazoe M.  Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells.  Cancer Res.   2004  64(9):3144-7  PubMed ID: 15126352  
2465  Sonoda E, Sasaki, M S, Morrison C, Yamaguchi-Iwai Y, Takata M, Takeda S  Sister chromatid exchanges are mediated by homologous recombination in vertebrate cells.  Mol Cell Biol  1999  19:5166-9  PubMed ID: 10373565  
2464  Takata M, Sasaki, M S, Sonoda E, Morrison C, Hashimoto M, Utsumi H, Yamaguchi-Iwai Y, Shinohara A, Takeda S  Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells.  EMBO J  1998  17:5497-508  PubMed ID: 9736627   DOI: 10.1093/emboj/17.18.5497
2463  Bezzubova O, Silbergleit A, Yamaguchi-Iwai Y, Takeda S, Buerstedde, J M  Reduced X-ray resistance and homologous recombination frequencies in a RAD54-/- mutant of the chicken DT40 cell line.  Cell  1997  89:185-93  PubMed ID: 9108474   DOI: 10.1016/s0092-8674(00)80198-1

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User's Publication
3086  Yin, Hong Lan, Suzuki, Yuka, Matsumoto, Yoshihisa, Tomita, Masanori, Furusawa, Yoshiya, Enomoto, Atsushi, Morita, Akinori, Aoki, Mizuho, Yatagai, Fumio, Suzuki, Takahiko, Hosoi, Yoshio, Ohtomo, Kuni, Suzuki, Norio  Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair.  Radiat Res  2004  162:433-41  PubMed ID: 15447039   DOI: 10.1667/rr3239



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