Cell No. : Cell Name
RCB1628 : ΔRad54-DT40
update : 2025/02/13
|
| Comment | Rad54 gene deleted DT40 (chicken B cell line). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takeda, Shunichi
|
| Originator |
Sonoda, Eiichiro
|
| Year of deposit |
2000
|
| Another name |
#33
|
| Original cell |
DT40
|
| Animal |
_avian
< Birds
|
| Tissue |
B cell
|
| Classification |
mutant
|
| Recombinant |
recombinant
|
| Exogene |
Rad54-hisD, Rad54-neo
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_1T34
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage cell No |
|
1.5-2 x 10 5 cells/ml
|
| Passage ratio |
|
1 : 10-20 split
|
| SC frequency |
|
Subculture : every 2 days
|
| Temperature |
|
39.5
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
90% FBS + 10% DMSO
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| DNA barcoding |
|
OK
|
| Others |
|
Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
|
| Reference information |
Reference |
6
|
| User's Publication |
1
|
| Reference |
|
17859
Zhao Z, Wu X, He F, Xiang C, Feng X, Bai X, Liu X, Zhao J, Takeda S, Qing Y.
Critical roles of Rad54 in tolerance to apigenin-induced Top1-mediated DNA damage
Exp Ther Med
2021
21(5):505
PubMed ID: 33791014
DOI: 10.3892/etm.2021.9936
|
|
17845
Sharma V, Collins LB, Chen TH, Herr N, Takeda S, Sun W, Swenberg JA, Nakamura J.
Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations
Oncotarget
2016
7(18):25377-90
PubMed ID: 27015367
DOI: 10.18632/oncotarget.8298
|
|
3435
Mizutani A, Okada T, Shibutani S, Sonoda E, Hochegger H, Nishigori C, Miyachi Y, Takeda S, Yamazoe M.
Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells.
Cancer Res.
2004
64(9):3144-7
PubMed ID: 15126352
DOI: 10.1158/0008-5472.can-03-3489
|
|
2465
Sonoda E, Sasaki, M S, Morrison C, Yamaguchi-Iwai Y, Takata M, Takeda S
Sister chromatid exchanges are mediated by homologous recombination in vertebrate cells.
Mol Cell Biol
1999
19:5166-9
PubMed ID: 10373565
DOI: 10.1128/MCB.19.7.5166
|
|
2464
Takata M, Sasaki, M S, Sonoda E, Morrison C, Hashimoto M, Utsumi H, Yamaguchi-Iwai Y, Shinohara A, Takeda S
Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells.
EMBO J
1998
17:5497-508
PubMed ID: 9736627
DOI: 10.1093/emboj/17.18.5497
|
|
2463
Bezzubova O, Silbergleit A, Yamaguchi-Iwai Y, Takeda S, Buerstedde, J M
Reduced X-ray resistance and homologous recombination frequencies in a RAD54-/- mutant of the chicken DT40 cell line.
Cell
1997
89:185-93
PubMed ID: 9108474
DOI: 10.1016/s0092-8674(00)80198-1
|
| User's Publication |
|
3086
Yin, Hong Lan, Suzuki, Yuka, Matsumoto, Yoshihisa, Tomita, Masanori, Furusawa, Yoshiya, Enomoto, Atsushi, Morita, Akinori, Aoki, Mizuho, Yatagai, Fumio, Suzuki, Takahiko, Hosoi, Yoshio, Ohtomo, Kuni, Suzuki, Norio
Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair.
Radiat Res
2004
162:433-41
PubMed ID: 15447039
DOI: 10.1667/rr3239
|
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