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Cell No. : Cell Name
RCB1621 : ΔKu70-DT40  update : 2022/04/05
CommentKu70 gene deleted DT40 (chicken B cell line).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takeda, Shunichi
Originator Takata, Minoru
Year of deposit 2000
Another name #77
Original cell DT40
Animal avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene Ku70-bsr, Ku70-his
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1T31
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10^(5) cells/ml
Passage ratio 1 : 10-20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information Reference 4
User's Publication 1

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3435  Mizutani A, Okada T, Shibutani S, Sonoda E, Hochegger H, Nishigori C, Miyachi Y, Takeda S, Yamazoe M.  Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells.  Cancer Res.   2004  64(9):3144-7  PubMed ID: 15126352  
3553  Adachi N, Ishino T, Ishii Y, Takeda S, Koyama H.  DNA ligase IV-deficient cells are more resistant to ionizing radiation in the absence of Ku70: Implications for DNA double-strand break repair.  Proc Natl Acad Sci U S A  2001  98(21):12109-13  PubMed ID: 11593023   DOI: 10.1073/pnas.201271098
2465  Sonoda E, Sasaki, M S, Morrison C, Yamaguchi-Iwai Y, Takata M, Takeda S  Sister chromatid exchanges are mediated by homologous recombination in vertebrate cells.  Mol Cell Biol  1999  19:5166-9  PubMed ID: 10373565  
2460  Sonoda E, Sasaki, M S, Buerstedde, J M, Bezzubova O, Shinohara A, Ogawa H, Takata M, Yamaguchi-Iwai Y, Takeda S  Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death.  EMBO J  1998  17:598-608  PubMed ID: 9430650  

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User's Publication
3086  Yin, Hong Lan, Suzuki, Yuka, Matsumoto, Yoshihisa, Tomita, Masanori, Furusawa, Yoshiya, Enomoto, Atsushi, Morita, Akinori, Aoki, Mizuho, Yatagai, Fumio, Suzuki, Takahiko, Hosoi, Yoshio, Ohtomo, Kuni, Suzuki, Norio  Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair.  Radiat Res  2004  162:433-41  PubMed ID: 15447039   DOI: 10.1667/rr3239

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