Cell No. : Cell Name RCB1621 : ΔKu70-DT40  update : 2024/11/13
Comment			Ku70 gene deleted DT40 (chicken B cell line).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Takeda, Shunichi
		Originator	Takata, Minoru
		Year of deposit	2000
		Another name	#77
		Original cell	DT40
		Animal	_avian < Birds
		Tissue	B cell
		Classification	mutant
		Recombinant	recombinant
		Exogene	Ku70-bsr, Ku70-his
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_1T31
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage cell No		1.5-2 x 10 5 cells/ml
		Passage ratio		1 : 10-20 split
		SC frequency		Subculture : every 2 days
		Temperature		39.5 ℃
		CO2 concentration		5 %
		Freeze medium		90% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Others		Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information		Reference	5
		User's Publication	1

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Reference

17845  Sharma V, Collins LB, Chen TH, Herr N, Takeda S, Sun W, Swenberg JA, Nakamura J.  Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations  Oncotarget  2016  7(18):25377-90  PubMed ID: 27015367   DOI: 10.18632/oncotarget.8298

3435  Mizutani A, Okada T, Shibutani S, Sonoda E, Hochegger H, Nishigori C, Miyachi Y, Takeda S, Yamazoe M.  Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells.  Cancer Res.   2004  64(9):3144-7  PubMed ID: 15126352   DOI: 10.1158/0008-5472.can-03-3489

3553  Adachi N, Ishino T, Ishii Y, Takeda S, Koyama H.  DNA ligase IV-deficient cells are more resistant to ionizing radiation in the absence of Ku70: Implications for DNA double-strand break repair.  Proc Natl Acad Sci U S A  2001  98(21):12109-13  PubMed ID: 11593023   DOI: 10.1073/pnas.201271098

2465  Sonoda E, Sasaki, M S, Morrison C, Yamaguchi-Iwai Y, Takata M, Takeda S  Sister chromatid exchanges are mediated by homologous recombination in vertebrate cells.  Mol Cell Biol  1999  19:5166-9  PubMed ID: 10373565   DOI: 10.1128/MCB.19.7.5166

2460  Sonoda E, Sasaki, M S, Buerstedde, J M, Bezzubova O, Shinohara A, Ogawa H, Takata M, Yamaguchi-Iwai Y, Takeda S  Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death.  EMBO J  1998  17:598-608  PubMed ID: 9430650   DOI: 10.1093/emboj/17.2.598

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User's Publication

3086  Yin, Hong Lan, Suzuki, Yuka, Matsumoto, Yoshihisa, Tomita, Masanori, Furusawa, Yoshiya, Enomoto, Atsushi, Morita, Akinori, Aoki, Mizuho, Yatagai, Fumio, Suzuki, Takahiko, Hosoi, Yoshio, Ohtomo, Kuni, Suzuki, Norio  Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair.  Radiat Res  2004  162:433-41  PubMed ID: 15447039   DOI: 10.1667/rr3239