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Cell No. : Cell Name
RCB1618 : HFLI-AE-VII  update : 2022/04/05
CommentHuman fibroblasts derived from liver of anencephalic fetus (17 weekly age). RCB1615 and RCB1617 are derived from the same fetus.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, I. & Ishikawa, H
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling embryo
Tissue embryo/fetus, liver
Classification normal
Year of origin 1999
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5512
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 0


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