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Cell No. : Cell Name
RCB1611 : M4  update : 2022/04/05
CommentAnti-chicken IgM antibody secreting. Able to stimulate DT40 cell surface antigen.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kurosaki, Tomohiro
Originator Cooper, Max
Year of deposit 1999
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U812
hybridoma Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3-x63-Ag8.653
Fusion_cell_2 strain mouse
Antibody name M4
Subclass IgM
Antigen_name chicken IgM
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 (4mM Glutamine) + 10% FBS + 0.1mM NEAA + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-15 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgM
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
2413  Chen, C L, Lehmeyer, J E, Cooper, M D  Evidence for an IgD homologue on chicken lymphocytes.  J Immunol  1982  129:2580-5  PubMed ID: 6183338  

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User's Publication
15982  Matthews SA, San Lek H, Morrison VL, Mackenzie MG, Zarrouk M, Cantrell D, Fagerholm SC.  Protein kinase D isoforms are dispensable for integrin-mediated lymphocyte adhesion and homing to lymphoid tissues  Eur J Immunol  2012  42(5):1316-26  PubMed ID: 22311617   DOI: 10.1002/eji.201142004



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