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Cell No. : Cell Name
RCB1609 : DHA9-15  update : 2021/01/08
CommentMouse T cell hybridoma (DO-11.10)expressing double HA-tagged HS1BP3 (HS1 SH3 domain binding protein).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Takemoto, Yoshihiro
Originator Takemoto, Yoshihiro
Year of deposit 1999
Original cell DO-11.10 (T cell hybridoma)
Animal mouse x mouse < Hybrid
Tissue T cell hybridoma
Classification hybrid cells
Recombinant recombinant
Exogene Double HA-tagged HS1BP3
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 100μg/ml G418
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 0

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4021  Takemoto Y, Furuta M, Sato M, Kubo M, Hashimoto Y.  Isolation and characterization of a novel HS1 SH3 domain binding protein, HS1BP3.  Int Immunol  1999  11:1957-1964  PubMed ID: 10590261

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