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Cell No. : Cell Name
RCB1598 : NCKA  update : 2024/01/17
CommentMouse T cell line transformed by Gross leukemia virus.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takemoto, Yoshihiro
Originator Hashimoto, Yasuhiro
Year of deposit 1999
Animal _mouse < Mammals
Strain name H-2k
Tissue T cell
Classification transformed
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_J696
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10mM HEPES + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 15 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0

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4020  Punt JA, Kubo RT, Saito T, Finkel TH, Kathiresan S, Blank KJ, Hashimoto Y.  Surface expression of a T cell receptor beta (TCR-beta) chain in the absence of TCR-alpha, -delta, and -gamma proteins.  J Exp Med  1991  174:775-783  PubMed ID: 1717631   DOI: 10.1084/jem.174.4.775
4019  Hashimoto Y, Blank KJ.  T cell receptor genes and T cell development in virus-transformed early T cell lines.  J Immunol  1990  44:1518-1525  PubMed ID: 2303715  

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