Cell No. : Cell Name
RCB1597 : KKC
update : 2024/01/17
|
| Comment | Mouse T cell line transformed by Gross leukemia virus. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takemoto, Yoshihiro
|
| Originator |
Hashimoto, Yasuhiro
|
| Year of deposit |
1999
|
| Animal |
_mouse
< Mammals
|
| Strain name |
H-2k
|
| Tissue |
T cell
|
| Classification |
transformed
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_J856
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10mM HEPES + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
|
1 : 15 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
4021
Takemoto Y, Furuta M, Sato M, Kubo M, Hashimoto Y.
Isolation and characterization of a novel HS1 SH3 domain binding protein, HS1BP3.
Int Immunol
1999
11:1957-1964
PubMed ID: 10590261
DOI: 10.1093/intimm/11.12.1957
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4020
Punt JA, Kubo RT, Saito T, Finkel TH, Kathiresan S, Blank KJ, Hashimoto Y.
Surface expression of a T cell receptor beta (TCR-beta) chain in the absence of TCR-alpha, -delta, and -gamma proteins.
J Exp Med
1991
174:775-783
PubMed ID: 1717631
DOI: 10.1084/jem.174.4.775
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4019
Hashimoto Y, Blank KJ.
T cell receptor genes and T cell development in virus-transformed early T cell lines.
J Immunol
1990
44:1518-1525
PubMed ID: 2303715
|
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