Cell No. : Cell Name
RCB1593 : OS3
update : 2026/03/27
|
| Comment | ori- SV40 DNA transformed C3H mouse brain-derived cells. GFAP+ astrocytes and GFAP- oligodendrocytes |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Sawada, Makoto
|
| Originator |
Asakura, Kunihiko
|
| Year of deposit |
1999
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H/HeN
|
| Gender |
Unknown
|
| Age at sampling |
2 days
|
| Tissue |
brain
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
pSV2 neo, ori-SV40
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| SDS |
SDS_DMSO(Japanese) 
|
| Cellosaurus(Expasy) |
CVCL_U177
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 0.2% D-Glucose + 10% FBS + 5μg/ml Bovine Insulin
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
3
|
| User's Publication |
5
|
| Reference |
|
2411
Asakura K, Suzumura A, Rodriguez M, Sawada M
Differentiation-specific mRNA expression of a mouse bipotential glial cell line.
Neurosci Lett
1998
258:21-4
PubMed ID: 9876042
DOI: 10.1016/s0304-3940(98)00862-3
|
|
2410
Sawamura S, Sawada M, Ito M, Nagatsu T, Nagatsu I, Suzumura A, Shibuya M, Sugita K, Marunouchi T
The bipotential glial progenitor cell line can develop into both oligodendrocytes and astrocytes in the mouse forebrain.
Neurosci Lett
1995
188:1-4
PubMed ID: 7783968
DOI: 10.1016/0304-3940(95)11378-a
|
|
2412
Ohtani K, Suzumura A, Sawada M, Marunouchi T, Nakashima I, Takahashi A
Establishment of mouse oligodendrocyte/type-2 astrocyte lineage cell line by transfection with origin-defective simian virus 40 DNA.
Cell Struct Funct
1992
17:325-33
PubMed ID: 1335367
DOI: 10.1247/csf.17.325
|
| User's Publication |
|
23766
Okubo T, Miyai M, Tomita H, Mori K, Niwa A, Imaizumi Y, Ichihashi K, Taniguchi T, Muto A, Suzuki H, Kanayama T, Sugie S, Izumo T, Hara A.
H3.3 G34R-engineered murine glioma reproduces diffuse perineuronal infiltration of diffuse hemispheric glioma, H3 G34-mutant.
Biochem Biophys Res Commun
2026
809:153471
PubMed ID: 41719882
DOI: 10.1016/j.bbrc.2026.153471
|
|
23191
Yamaura T, Watanabe M, Minato Y, Sakuma R, Maeda S, Tachibana T, Yagi H.
Laminin 332 regulates glial fibrillary acidic protein expression and astrocyte maturation during spinal cord development.
J Anat
2025
PubMed ID: 40930803
DOI: 10.1111/joa.70041
|
|
17080
Miyai M, Kanayama T, Hyodo F, Kinoshita T, Ishihara T, Okada H, Suzuki H, Takashima S, Wu Z, Hatano Y, Egashira Y, Enomoto Y, Nakayama N, Soeda A, Yano H, Hirata A, Niwa M, Sugie S, Mori T, Maekawa Y, Iwama T, Matsuo M, Hara A, Tomita H.
Glucose transporter Glut1 controls diffuse invasion phenotype with perineuronal satellitosis in diffuse glioma microenvironment
Neurooncol Adv
2020
3(1):vdaa150
PubMed ID: 33506198
DOI: 10.1093/noajnl/vdaa150
|
|
10102
Mahmoud ME, Fereig R, Nishikawa Y.
Involvement of Host Defense Mechanisms against Toxoplasma gondii Infection in Anhedonic and Despair-Like Behaviors in Mice.
Infect. Immun.
2017
85:
PubMed ID: 28138019
DOI: 10.1128/IAI.00007-17
|
|
9484
Masaki Tanaka, Yoshihisa Watanabe, Kanji Yoshimoto
Regulation of Relaxin 3 Gene Expression via cAMP-PKA in a Neuroblastoma Cell Line
J Neurosci Resc
2009
87(3):820-9
PubMed ID: 18831067
DOI: 10.1002/jnr.21895
|
Back
Top
CELL BANK Website