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Cell No. : Cell Name
RCB1593 : OS3  update : 2020/09/24
Commentori- SV40 DNA transformed C3H mouse brain-derived cells. GFAP+ astrocytes and GFAP- oligodendrocytes
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Sawada, Makoto
Originator Asakura, Kunihiko
Year of deposit 1999
Animal mouse < Mammals
Strain name C3H/HeN
Gender Unknown
Age at sampling 2 days
Tissue brain
Classification transformed
Recombinant recombinant
Exogene pSV2 neo, ori-SV40
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 0.2% D-Glucose + 10% FBS + 5μg/ml Bovine Insulin
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 2


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Reference
2411  Asakura K, Suzumura A, Rodriguez M, Sawada M  Differentiation-specific mRNA expression of a mouse bipotential glial cell line.  Neurosci Lett  1998  258:21-4  PubMed ID: 9876042
2410  Sawamura S, Sawada M, Ito M, Nagatsu T, Nagatsu I, Suzumura A, Shibuya M, Sugita K, Marunouchi T  The bipotential glial progenitor cell line can develop into both oligodendrocytes and astrocytes in the mouse forebrain.  Neurosci Lett  1995  188:1-4  PubMed ID: 7783968
2412  Ohtani K, Suzumura A, Sawada M, Marunouchi T, Nakashima I, Takahashi A  Establishment of mouse oligodendrocyte/type-2 astrocyte lineage cell line by transfection with origin-defective simian virus 40 DNA.  Cell Struct Funct  1992  17:325-33  PubMed ID: 1335367

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User's Publication
10102  Mahmoud ME, Fereig R, Nishikawa Y.  Involvement of Host Defense Mechanisms against Toxoplasma gondii Infection in Anhedonic and Despair-Like Behaviors in Mice.  Infect. Immun.  2017  85:  PubMed ID: 28138019
9484  Masaki Tanaka, Yoshihisa Watanabe, Kanji Yoshimoto  Regulation of Relaxin 3 Gene Expression via cAMP-PKA in a Neuroblastoma Cell Line  J Neurosci Resc  2009  87(3):820-9  PubMed ID: 18831067



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