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Cell No. : Cell Name
RCB1593 : OS3  update : 2020/09/24
Commentori- SV40 DNA transformed C3H mouse brain-derived cells. GFAP+ astrocytes and GFAP- oligodendrocytes
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Sawada, Makoto
Originator Asakura, Kunihiko
Year of deposit 1999
Animal mouse < Mammals
Strain name C3H/HeN
Gender Unknown
Age at sampling 2 days
Tissue brain
Classification transformed
Recombinant recombinant
Exogene pSV2 neo, ori-SV40
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 0.2% D-Glucose + 10% FBS + 5μg/ml Bovine Insulin
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
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lot info
Reference information Reference 3
User's Publication 2

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2411  Asakura K, Suzumura A, Rodriguez M, Sawada M  Differentiation-specific mRNA expression of a mouse bipotential glial cell line.  Neurosci Lett  1998  258:21-4  PubMed ID: 9876042
2410  Sawamura S, Sawada M, Ito M, Nagatsu T, Nagatsu I, Suzumura A, Shibuya M, Sugita K, Marunouchi T  The bipotential glial progenitor cell line can develop into both oligodendrocytes and astrocytes in the mouse forebrain.  Neurosci Lett  1995  188:1-4  PubMed ID: 7783968
2412  Ohtani K, Suzumura A, Sawada M, Marunouchi T, Nakashima I, Takahashi A  Establishment of mouse oligodendrocyte/type-2 astrocyte lineage cell line by transfection with origin-defective simian virus 40 DNA.  Cell Struct Funct  1992  17:325-33  PubMed ID: 1335367

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User's Publication
10102  Mahmoud ME, Fereig R, Nishikawa Y.  Involvement of Host Defense Mechanisms against Toxoplasma gondii Infection in Anhedonic and Despair-Like Behaviors in Mice.  Infect. Immun.  2017  85:  PubMed ID: 28138019
9484  Masaki Tanaka, Yoshihisa Watanabe, Kanji Yoshimoto  Regulation of Relaxin 3 Gene Expression via cAMP-PKA in a Neuroblastoma Cell Line  J Neurosci Resc  2009  87(3):820-9  PubMed ID: 18831067

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