細胞番号 : 細胞名 RCB1591 : SRA 01/04  update : 2024/08/14
細胞特性(Comment:英)			alpha, beta-crystaline expressing human lense epithelial cell line. plasmid DNA: pRSV-B-T Ag.
細胞特性(日)			ヒト水晶体上皮細胞株。αβクリスタリンを発現する。導入遺伝子は、SV40 T遺伝子(pRSV-B-T Ag)。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)			基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Ibaraki, Nobuhiro
		樹立者	Ibaraki, Nobuhiro
		寄託日	1999
		動物種	_human < Mammals
		属名	Homo
		種名	sapiens
		性別	Male
		齢	3 months
		採取組織	eye, lens
		細胞分類	transformed
		遺伝子改変	recombinant
		外来遺伝子	pRSV-B-T Ag
		細胞寿命	infinite
		細胞形態	epithelial-like
		Cellosaurus(Expasy)	CVCL_7157
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		DMEM (low glucose) + 20% FBS
		抗生物質		Free
		継代方法		0.25% Trypsin
		継代密度		1 : 8 split
		継代・培地交換頻度		Subculture : once/week, Medium Renewal : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ/アコレプラズマ		(-)
		アイソザイム検査		LD, NP
		染色体検査		55-60(50) : 55(2),56(9),57(12),58(15),59(10),60(2)
		個体識別検査		OK
画像情報			寄託時情報	ロット情報
文献情報		Reference(英)	4件
		Reference(日)	0件
		利用者成果(英)	29件
		利用者成果(日)	0件

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Reference(英)

4971  Yamashiro Y1, Sasaki H, Ibaraki N, Nagai K, Kawakami Y, Yaguchi H, Fujita N, Osada H, Sasaki K.  Cyclin-dependent kinase inhibitor p16 and p21 expression, and cell cycle change in human lens epithelial cell line SRA 01/04 following contact inhibition in normal culture.  Ophthalmic Res  2011  46(1):38-43  PubMed ID: 21212707   DOI: 10.1159/000322805

4970  Oharazawa H, Ibaraki N, Ohara K, Reddy VN.  Inhibitory effects of Arg-Gly-Asp (RGD) peptide on cell attachment and migration in a human lens epithelial cell line.  Ophthalmic Res  2005  37(4):191-6  PubMed ID: 15990462   DOI: 10.1159/000086595

4969  Oharazawa H, Ibaraki N, Lin LR, Reddy VN.  The effects of extracellular matrix on cell attachment, proliferation and migration in a human lens epithelial cell line.  Exp Eye Res  1999  69(6):603-10  PubMed ID: 10620389   DOI: 10.1006/exer.1999.0723

2409  Ibaraki N, Chen, S C, Lin, L R, Okamoto H, Pipas, J M, Reddy, V N  Human Lens Epithelial Cell Line  Exp Cell Res  1998  67:577-585  PubMed ID: 9878220   DOI: 10.1006/exer.1998.0551

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Reference(日)

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利用者成果(英)

19884  Li D, Che X, Gao N, Li J.  CircSTRBP contributes to H2O2-induced lens epithelium cell dysfunction through increasing NOX4 mRNA stability by recruiting IGF2BP1  Exp Eye Res  2024  109817  PubMed ID: 38340945   DOI: 10.1016/j.exer.2024.109817

21828  Cui Y, Yu X, Bao J, Ping X, Shi S, Huang Y, Yin Q, Yang H, Chen R, Yao K, Chen X, Shentu X.  Lens autophagy protein ATG16L1: a potential target for cataract treatment.  Theranostics  2024  14(10):3984-3996  PubMed ID: 38994020   DOI: 10.7150/thno.93864

22119  Wei YS, Liu HR, Yang Q, Zhi Z, Yu Y.  Anp32b-deficiency suppresses ocular development by repression of Pax6.  Ophthalmic Res  2024  1-13  PubMed ID: 39504945   DOI: 10.1159/000542447

19641  Wu J, Chen S, Xu J, Xu W, Zheng S, Tian Q, Luo C, Chen X, Shentu X.  Insight into Pathogenic Mechanism Underlying the Hereditary Cataract Caused by βB2-G149V Mutation  Biomolecules  2023  13(5):864  PubMed ID: 37238733   DOI: 10.3390/biom13050864

21047  Takeda S, Yamamoto N, Nagai N, Hiramatsu N, Deguchi S, Hatsusaka N, Kubo E, Sasaki H.  Function of mitochondrial cytochrome c oxidase is enhanced in human lens epithelial cells at high temperatures.  Mol Med Rep  2023  27(1)  PubMed ID: 36484359   DOI: 10.3892/mmr.2022.12906

21293  Huang Y, Ping X, Cui Y, Yang H, Bao J, Yin Q, Ailifeire H, Shentu X.  Glycolysis Aids in Human Lens Epithelial Cells' Adaptation to Hypoxia.  Antioxidants (Basel)  2023  12(6):10.3390/antiox12061304  PubMed ID: 37372033   DOI: 10.3390/antiox12061304

20872  Kang L, Luo J, Li P, Zhang G, Wei M, Ji M, Guan H.  miR-125a-3p regulates apoptosis by suppressing TMBIM4 in lens epithelial cells.  Int Ophthalmol  2022    PubMed ID: 36173547   DOI: 10.1007/s10792-022-02524-x

20915  Luo J, Li P, Kang L, Ji M, Zhou T, Qin B, Zhang J, Guan H.  Exosomal microRNA-222-3p increases UVB sensitivity of lens epithelium cells by suppressing MGMT.  Int Ophthalmol  2022    PubMed ID: 36319884   DOI: 10.1007/s10792-022-02560-7

17335  Shih CC, Lee CY, Wong FF, Lin CH.  Protective Effects of One 2,4-Dihydro-3H-Pyrazol-3-one Derivative against Posterior Capsular Opacification by Regulation of TGF-β2/SMADs and Non-SMAD Signaling, Collagen I, and Fibronectin Proteins  Curr Issues Mol Biol  2022  44(10):5048-5066  PubMed ID: 36286058   DOI: 10.3390/cimb44100343

21147  Guo G, Dong J.  Diosmetin attenuates oxidative stress-induced damage to lens epithelial cells via the mitogen-activated protein kinase (MAPK) pathway.  Bioengineered  2022  13(4):11072-11081  PubMed ID: 35481411   DOI: 10.1080/21655979.2022.2068755

20092  Ping X, Cheng Y, Bao J, Shi K, Zou J, Shentu X.  KPNA4 is involved in cataract formation via the nuclear import of p53.  Gene  2021  145621  PubMed ID: 33798680   DOI: 10.1016/j.gene.2021.145621

15154  Ping X, Liang J, Shi K, Bao J, Wu J, Yu X, Tang X, Zou J, Shentu X.  Rapamycin relieves the cataract caused by ablation of Gja8b through stimulating autophagy in zebrafish  Autophagy  2021  1-15  PubMed ID: 33472493   DOI: 10.1080/15548627.2021.1872188

16797  Zhang G, Zhang M, Yu J, Kang L, Guan H.  Ginsenoside Rg1 Prevents H 2 O 2-induced Lens Opacity  Curr Eye Res  2021  46(8):1159-1165  PubMed ID: 33405968   DOI: 10.1080/02713683.2020.1869266

13564  Yamamoto N, Takeda S, Hatsusaka N, Hiramatsu N, Nagai N, Deguchi S, Nakazawa Y, Takata T, Kodera S, Hirata A, Kubo E, Sasaki H.  Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)  Cells  2020  9(12):2670  PubMed ID: 33322631   DOI: 10.3390/cells9122670

13935  Cui YL, Yu XN, Zhang X, Tang YL, Tang XJ, Yang H, Ping XY, Wu J, Yin QC, Zhou JY, Xu XY, Shentu XC.  Association of IGF1R polymorphisms (rs1546713) with susceptibility to age-related cataract in a Han Chinese population  Int J Ophthalmol  2020  13(3):374-381.  PubMed ID: 32309172   DOI: 10.18240/ijo.2020.03.02

15095  Chen X, Li P, Zhang G, Kang L, Qin B, Mao X, Qin M, Cao Y, Wang Y, Guan H.  Comprehensive Profiling of Proteome and Ubiquitome Changes in Human Lens Epithelial Cell Line after Ultraviolet-B Irradiation  ACS Omega  2020  5(50):32171-32182  PubMed ID: 33376855   DOI: 10.1021/acsomega.0c03088

16818  Ganoderic acid A protects lens epithelial cells from UVB irradiation and delays lens opacity  Ganoderic acid A protects lens epithelial cells from UVB irradiation and delays lens opacity  Chin J Nat Med  2020  18(12):934-940  PubMed ID: 33357724   DOI: 10.1016/S1875-5364(20)60037-1

11595  Koyama S, Narita E, Suzuki Y, Shiina T, Taki M, Shinohara N, Miyakoshi J.  Long-term exposure to a 40-GHz electromagnetic field does not affect genotoxicity or heat shock protein expression in HCE-T or SRA01/04 cells.  J. Radiat. Res.  2019    PubMed ID: 31197350   DOI: 10.1093/jrr/rrz017

10720  Shentu XC, Ping XY, Cheng YL, Zhang X, Tang YL, Tang XJ.  Hydrogen peroxide-induced apoptosis of human lens epithelial cells is inhibited by parthenolide.  Int J Ophthalmol  2018  11:12-17  PubMed ID: 29375984   DOI: 10.18240/ijo.2018.01.03

11257  Yu X, Ping X, Zhang X, Cui Y, Yang H, Tang X, Tang Y, Shentu X.  The impact of GJA8 SNPs on susceptibility to age-related cataract.  Hum. Genet.  2018    PubMed ID: 30349978   DOI: 10.1007/s00439-018-1945-5

9179  Qiuli Fu, Zhenwei Qin, Lifang Zhang, Danni Lyu, Qiaomei Tang, Houfa Yin, Zhijian Chen, Ke Yao  A New Long Noncoding RNA ALB Regulates Autophagy by Enhancing the Transformation of LC3BI to LC3BII During Human Lens Development  Mol Ther Nucleic Acids  2017  9, 207-217  PubMed ID: 29246299   DOI: 10.1016/j.omtn.2017.09.011

9519  Zhu K, Lv Y, Cheng Q, Hua J, Zeng Q.  Extremely Low Frequency Magnetic Fields Do Not Induce DNA Damage in Human Lens Epithelial Cells In Vitro.  Anat Rec (Hoboken)  2016  299:688-97  PubMed ID: 27079842   DOI: 10.1002/ar.23312

9778  Koyama S, Narita E, Shimizu Y, Suzuki Y, Shiina T, Taki M, Shinohara N, Miyakoshi J.  Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye.  Int J Environ Res Public Health  2016  13:  PubMed ID: 27509516   DOI: 10.3390/ijerph13080802

17134  Shi D, Zhu C, Lu R, Mao S, Qi Y.  Intermediate frequency magnetic field generated by a wireless power transmission device does not cause genotoxicity in vitro  Bioelectromagnetics  2014  35(7):512-8  PubMed ID: 25196478   DOI: 10.1002/bem.21872

15677  Xu S, Chen G, Chen C, Sun C, Zhang D, Murbach M, Kuster N, Zeng Q, Xu Z.  Cell type-dependent induction of DNA damage by 1800 MHz radiofrequency electromagnetic fields does not result in significant cellular dysfunctions  PLoS One  2013  e54906  PubMed ID: 23355902   DOI: 10.1371/journal.pone.0054906

15754  Hori K, Matsuda A, Ebihara N, Imai K, Mori K, Funaki T, Watanabe Y, Nakatani S, Okada K, Matsuo O, Murakami A.  Involvement of plasminogen activator inhibitor-1 in the pathogenesis of atopic cataracts  Invest Ophthalmol Vis Sci  2012  53(4):1846-51  PubMed ID: 22410565   DOI: 10.1167/iovs.11-8380

8076  Tang X, Yao K, Zhang L, Yang Y, Yao H.  Honokiol inhibits H(2)O(2)-induced apoptosis in human lens epithelial cells via inhibition of the mitogen-activated protein kinase and Akt pathways.  Eur. J. Pharmacol.  2011  650:72-8  PubMed ID: 20965163   DOI: 10.1016/j.ejphar.2010.09.076

15646  Kaji Y, Kiuchi T, Oshika T.  Carteolol hydrochloride suppresses the generation of reactive oxygen species and rescues cell death after ultraviolet irradiation of cultured lens epithelial cells  Open Ophthalmol J  2010  4:60-5  PubMed ID: 21283534   DOI: 10.2174/1874364101004010060

18687  Yao K, Zhang L, Zhang Y, Ye P, Zhu N.  The flavonoid, fisetin, inhibits UV radiation-induced oxidative stress and the activation of NF-kappaB and MAPK signaling in human lens epithelial cells  Mol Vis  2008  14:1865-7  PubMed ID: 18949064

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