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Cell No. : Cell Name
RCB1591 : SRA 01/04  update : 2022/04/05
Commentalpha, beta-crystaline expressing human lense epithelial cell line. plasmid DNA: pRSV-B-T Ag.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ibaraki, Nobuhiro
Originator Ibaraki, Nobuhiro
Year of deposit 1999
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 3 months
Tissue eye, lens
Classification transformed
Recombinant recombinant
Exogene pRSV-B-T Ag
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_7157
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 20% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 55-60(50) : 55(2),56(9),57(12),58(15),59(10),60(2)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 19


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Reference
4971  Yamashiro Y1, Sasaki H, Ibaraki N, Nagai K, Kawakami Y, Yaguchi H, Fujita N, Osada H, Sasaki K.  Cyclin-dependent kinase inhibitor p16 and p21 expression, and cell cycle change in human lens epithelial cell line SRA 01/04 following contact inhibition in normal culture.  Ophthalmic Res  2011  46(1):38-43  PubMed ID: 21212707   DOI: 10.1159/000322805
4970  Oharazawa H, Ibaraki N, Ohara K, Reddy VN.  Inhibitory effects of Arg-Gly-Asp (RGD) peptide on cell attachment and migration in a human lens epithelial cell line.  Ophthalmic Res  2005  37(4):191-6  PubMed ID: 15990462   DOI: 10.1159/000086595
4969  Oharazawa H, Ibaraki N, Lin LR, Reddy VN.  The effects of extracellular matrix on cell attachment, proliferation and migration in a human lens epithelial cell line.  Exp Eye Res  1999  69(6):603-10  PubMed ID: 10620389   DOI: 10.1006/exer.1999.0723
2409  Ibaraki N, Chen, S C, Lin, L R, Okamoto H, Pipas, J M, Reddy, V N  Human Lens Epithelial Cell Line  Exp Cell Res  1998  67:577-585  PubMed ID: 9878220   DOI: 10.1006/exer.1998.0551

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User's Publication
20872  Kang L, Luo J, Li P, Zhang G, Wei M, Ji M, Guan H.  miR-125a-3p regulates apoptosis by suppressing TMBIM4 in lens epithelial cells.  Int Ophthalmol  2022    PubMed ID: 36173547   DOI: 10.1007/s10792-022-02524-x
20915  Luo J, Li P, Kang L, Ji M, Zhou T, Qin B, Zhang J, Guan H.  Exosomal microRNA-222-3p increases UVB sensitivity of lens epithelium cells by suppressing MGMT.  Int Ophthalmol  2022    PubMed ID: 36319884   DOI: 10.1007/s10792-022-02560-7
20092  Ping X, Cheng Y, Bao J, Shi K, Zou J, Shentu X.  KPNA4 is involved in cataract formation via the nuclear import of p53.  Gene  2021  145621  PubMed ID: 33798680   DOI: 10.1016/j.gene.2021.145621
15154  Ping X, Liang J, Shi K, Bao J, Wu J, Yu X, Tang X, Zou J, Shentu X.  Rapamycin relieves the cataract caused by ablation of Gja8b through stimulating autophagy in zebrafish  Autophagy  2021  1-15  PubMed ID: 33472493   DOI: 10.1080/15548627.2021.1872188
16797  Zhang G, Zhang M, Yu J, Kang L, Guan H.  Ginsenoside Rg1 Prevents H 2 O 2-induced Lens Opacity  Curr Eye Res  2021  46(8):1159-1165  PubMed ID: 33405968   DOI: 10.1080/02713683.2020.1869266
13564  Yamamoto N, Takeda S, Hatsusaka N, Hiramatsu N, Nagai N, Deguchi S, Nakazawa Y, Takata T, Kodera S, Hirata A, Kubo E, Sasaki H.  Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)  Cells  2020  9(12):2670  PubMed ID: 33322631   DOI: 10.3390/cells9122670
13935  Cui YL, Yu XN, Zhang X, Tang YL, Tang XJ, Yang H, Ping XY, Wu J, Yin QC, Zhou JY, Xu XY, Shentu XC.  Association of IGF1R polymorphisms (rs1546713) with susceptibility to age-related cataract in a Han Chinese population  Int J Ophthalmol  2020  13(3):374-381.  PubMed ID: 32309172   DOI: 10.18240/ijo.2020.03.02
15095  Chen X, Li P, Zhang G, Kang L, Qin B, Mao X, Qin M, Cao Y, Wang Y, Guan H.  Comprehensive Profiling of Proteome and Ubiquitome Changes in Human Lens Epithelial Cell Line after Ultraviolet-B Irradiation  ACS Omega  2020  5(50):32171-32182  PubMed ID: 33376855   DOI: 10.1021/acsomega.0c03088
16818  Ganoderic acid A protects lens epithelial cells from UVB irradiation and delays lens opacity  Ganoderic acid A protects lens epithelial cells from UVB irradiation and delays lens opacity  Chin J Nat Med  2020  18(12):934-940  PubMed ID: 33357724   DOI: 10.1016/S1875-5364(20)60037-1
11595  Koyama S, Narita E, Suzuki Y, Shiina T, Taki M, Shinohara N, Miyakoshi J.  Long-term exposure to a 40-GHz electromagnetic field does not affect genotoxicity or heat shock protein expression in HCE-T or SRA01/04 cells.  J. Radiat. Res.  2019    PubMed ID: 31197350   DOI: 10.1093/jrr/rrz017
10720  Shentu XC, Ping XY, Cheng YL, Zhang X, Tang YL, Tang XJ.  Hydrogen peroxide-induced apoptosis of human lens epithelial cells is inhibited by parthenolide.  Int J Ophthalmol  2018  11:12-17  PubMed ID: 29375984   DOI: 10.18240/ijo.2018.01.03
11257  Yu X, Ping X, Zhang X, Cui Y, Yang H, Tang X, Tang Y, Shentu X.  The impact of GJA8 SNPs on susceptibility to age-related cataract.  Hum. Genet.  2018    PubMed ID: 30349978   DOI: 10.1007/s00439-018-1945-5
9179  Qiuli Fu, Zhenwei Qin, Lifang Zhang, Danni Lyu, Qiaomei Tang, Houfa Yin, Zhijian Chen, Ke Yao  A New Long Noncoding RNA ALB Regulates Autophagy by Enhancing the Transformation of LC3BI to LC3BII During Human Lens Development  Mol Ther Nucleic Acids  2017  9, 207-217  PubMed ID: 29246299   DOI: 10.1016/j.omtn.2017.09.011
9519  Zhu K, Lv Y, Cheng Q, Hua J, Zeng Q.  Extremely Low Frequency Magnetic Fields Do Not Induce DNA Damage in Human Lens Epithelial Cells In Vitro.  Anat Rec (Hoboken)  2016  299:688-97  PubMed ID: 27079842   DOI: 10.1002/ar.23312
17134  Shi D, Zhu C, Lu R, Mao S, Qi Y.  Intermediate frequency magnetic field generated by a wireless power transmission device does not cause genotoxicity in vitro  Bioelectromagnetics  2014  35(7):512-8  PubMed ID: 25196478   DOI: 10.1002/bem.21872
15677  Xu S, Chen G, Chen C, Sun C, Zhang D, Murbach M, Kuster N, Zeng Q, Xu Z.  Cell type-dependent induction of DNA damage by 1800 MHz radiofrequency electromagnetic fields does not result in significant cellular dysfunctions  PLoS One  2013  e54906  PubMed ID: 23355902   DOI: 10.1371/journal.pone.0054906
15754  Hori K, Matsuda A, Ebihara N, Imai K, Mori K, Funaki T, Watanabe Y, Nakatani S, Okada K, Matsuo O, Murakami A.  Involvement of plasminogen activator inhibitor-1 in the pathogenesis of atopic cataracts  Invest Ophthalmol Vis Sci  2012  53(4):1846-51  PubMed ID: 22410565   DOI: 10.1167/iovs.11-8380
8076  Tang X, Yao K, Zhang L, Yang Y, Yao H.  Honokiol inhibits H(2)O(2)-induced apoptosis in human lens epithelial cells via inhibition of the mitogen-activated protein kinase and Akt pathways.  Eur. J. Pharmacol.  2011  650:72-8  PubMed ID: 20965163   DOI: 10.1016/j.ejphar.2010.09.076
15646  Kaji Y, Kiuchi T, Oshika T.  Carteolol hydrochloride suppresses the generation of reactive oxygen species and rescues cell death after ultraviolet irradiation of cultured lens epithelial cells  Open Ophthalmol J  2010  4:60-5  PubMed ID: 21283534   DOI: 10.2174/1874364101004010060



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