Cell No. : Cell Name RCB1560 : PWS-Yamaguchi  update : 2022/04/05
Comment			Prader-Willi syndrome with obesity. del(15) was found in lymphocytes of the patient.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Nishi, Yoshihiro
		Originator	Nishi, Yoshihiro
		Year of deposit	1999
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	26 years
		Tissue	skin
		Genetics	Prader-Willi syndrome
		Classification	normal
		Year of origin	1999
		Lifespan	finite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_L556
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM/HamF12 + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 1-2 times/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Isozyme		LD, NP
		Chromosome mode		46(50)
		STR(human)		OK
Reference information		Reference	0
		User's Publication	3

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Reference

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User's Publication

20231  Sanderson MR, Fahlman RP, Wevrick R.  The N-terminal domain of the Schaaf-Yang syndrome protein MAGEL2 likely has a role in RNA metabolism.  J Biol Chem  2021    PubMed ID: 34265304   DOI: 10.1016/j.jbc.2021.100959

8172  Bush JR, Wevrick R.  Loss of Necdin impairs myosin activation and delays cell polarization.  Genesis  2010  48:540-53  PubMed ID: 20665884   DOI: 10.1002/dvg.20658

3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504