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Cell No. : Cell Name
RCB1560 : PWS-Yamaguchi  update : 2022/04/05
CommentPrader-Willi syndrome with obesity. del(15) was found in lymphocytes of the patient.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishi, Yoshihiro
Originator Nishi, Yoshihiro
Year of deposit 1999
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 26 years
Tissue skin
Genetics Prader-Willi syndrome
Classification normal
Year of origin 1999
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_L556
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 46(50)
STR(human) OK
Reference information Reference 1
User's Publication 3


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Reference
2458  Kobayashi M, Sasaoka T, Takata Y, Hisatomi A, Shigeta Y  Insulin resistance by uncleaved insulin proreceptor. Emergence of binding site by trypsin.  Diabetes  1988  37:653-6  PubMed ID: 3360220  

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User's Publication
20231  Sanderson MR, Fahlman RP, Wevrick R.  The N-terminal domain of the Schaaf-Yang syndrome protein MAGEL2 likely has a role in RNA metabolism.  J Biol Chem  2021    PubMed ID: 34265304   DOI: 10.1016/j.jbc.2021.100959
8172  Bush JR, Wevrick R.  Loss of Necdin impairs myosin activation and delays cell polarization.  Genesis  2010  48:540-53  PubMed ID: 20665884   DOI: 10.1002/dvg.20658
3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504



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