Cell No. : Cell Name
RCB1555 : F9
update : 2023/03/09
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| Comment | Said to differentiate to parietal endoderm with retinoic acid and dibutyryl cAMP. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Shanghai Cell Bank
|
| Year of deposit |
1999
|
| Animal |
mouse
< Mammals
|
| Strain name |
129
|
| Age at sampling |
embryo
|
| Tissue |
embryo
|
| Disease name |
Embryonal carcinoma
|
| Classification |
cancer
|
| History |
Cong Xiao-qian (Shanghai Inst. of Cell Biol.) -> Type Culture Collection of Chinese Academy of Scie
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_0259
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|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
RPMI1640 + 20% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
pipetting
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/2 weeks, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
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| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
2
|
| Reference |
|
2493
Strickland S, Mahdavi V.
The induction of differentiation in teratocarcinoma stem cells by retinoic acid.
Cell
1978
15(2):393-403
PubMed ID: 214238
DOI: 10.1016/0092-8674(78)90008-9
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4967
Nicolas JF, Dubois P, Jakob H, Gaillard J, Jacob F.
Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)
Ann Microbiol (Paris)
1975
126(1):3-22
PubMed ID: 1103673
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844
Berstine EG, Hooper ML, Grandchamp S, Ephrussi B.
Alkaline phosphatase activity in mouse teratoma.
Proc Natl Acad Sci U S A
1973
70(12):3899-903
PubMed ID: 4521215
DOI: 10.1073/pnas.70.12.3899
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4966
Artzt K, Dubois P, Bennett D, Condamine H, Babinet C, Jacob F.
Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.
Proc Natl Acad Sci U S A
1973
70(10):2988-92
PubMed ID: 4355379
DOI: 10.1073/pnas.70.10.2988
|
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