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Cell No. : Cell Name
RCB1555 : F9  update : 2022/04/05
CommentSaid to differentiate to parietal endoderm with retinoic acid and dibutyryl cAMP.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Shanghai Cell Bank
Year of deposit 1999
Animal mouse < Mammals
Strain name 129
Age at sampling embryo
Tissue embryo
Disease name Embryonal carcinoma
Classification cancer
History Cong Xiao-qian (Shanghai Inst. of Cell Biol.) -> Type Culture Collection of Chinese Academy of Scie
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_0259
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 20% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 4
User's Publication 2


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Reference
2493  Strickland S, Mahdavi V.  The induction of differentiation in teratocarcinoma stem cells by retinoic acid.  Cell  1978  15(2):393-403  PubMed ID: 214238   DOI: 10.1016/0092-8674(78)90008-9
4967  Nicolas JF, Dubois P, Jakob H, Gaillard J, Jacob F.  Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)  Ann Microbiol (Paris)  1975  126(1):3-22  PubMed ID: 1103673  
844  Berstine EG, Hooper ML, Grandchamp S, Ephrussi B.  Alkaline phosphatase activity in mouse teratoma.  Proc Natl Acad Sci U S A  1973  70(12):3899-903  PubMed ID: 4521215   DOI: 10.1073/pnas.70.12.3899
4966  Artzt K, Dubois P, Bennett D, Condamine H, Babinet C, Jacob F.  Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.  Proc Natl Acad Sci U S A  1973  70(10):2988-92  PubMed ID: 4355379   DOI: 10.1073/pnas.70.10.2988

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User's Publication
11170  Nishioka K, Miyazaki H, Soejima H.  Unbiased shRNA screening, using a combination of FACS and high-throughput sequencing, enables identification of novel modifiers of Polycomb silencing.  Sci Rep  2018  8(1):12128  PubMed ID: 30108332   DOI: 10.1038/s41598-018-30649-6
8793  Mezaki Y, Yoshikawa K, Yamaguchi N, Miura M, Imai K, Kato S, Senoo H.  Rat hepatic stellate cells acquire retinoid responsiveness after activation in vitro by post-transcriptional regulation of retinoic acid receptor alpha gene expression.  Arch. Biochem. Biophys.  2007  465:370-9  PubMed ID: 17693386   DOI: 10.1016/j.abb.2007.06.024



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