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Cell No. : Cell Name
RCB1555 : F9  update : 2023/03/09
CommentSaid to differentiate to parietal endoderm with retinoic acid and dibutyryl cAMP.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Shanghai Cell Bank
Year of deposit 1999
Animal _mouse < Mammals
Strain name 129
Age at sampling embryo
Tissue embryo
Disease name Embryonal carcinoma
Classification cancer
History Cong Xiao-qian (Shanghai Inst. of Cell Biol.) -> Type Culture Collection of Chinese Academy of Scie
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_0259
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 20% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 6
User's Publication 3

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2593  Hogan BL, Taylor A, Adamson E.  Cell interactions modulate embryonal carcinoma cell differentiation into parietal or visceral endoderm  Nature  1981  291(5812):235-7  PubMed ID: 6164928   DOI: 10.1038/291235a0
2592  Strickland S, Smith KK, Marotti KR.  Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP  Cell  1980  21(2):347-55.  PubMed ID: 6250719   DOI: 10.1016/0092-8674(80)90471-7
2493  Strickland S, Mahdavi V.  The induction of differentiation in teratocarcinoma stem cells by retinoic acid.  Cell  1978  15(2):393-403  PubMed ID: 214238   DOI: 10.1016/0092-8674(78)90008-9
4967  Nicolas JF, Dubois P, Jakob H, Gaillard J, Jacob F.  Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)  Ann Microbiol (Paris)  1975  126(1):3-22  PubMed ID: 1103673  
844  Berstine EG, Hooper ML, Grandchamp S, Ephrussi B.  Alkaline phosphatase activity in mouse teratoma.  Proc Natl Acad Sci U S A  1973  70(12):3899-903  PubMed ID: 4521215   DOI: 10.1073/pnas.70.12.3899
4966  Artzt K, Dubois P, Bennett D, Condamine H, Babinet C, Jacob F.  Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.  Proc Natl Acad Sci U S A  1973  70(10):2988-92  PubMed ID: 4355379   DOI: 10.1073/pnas.70.10.2988

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User's Publication
21635  Noguchi Y, Onodera Y, Miyamoto T, Maruoka M, Kosako H, Suzuki J.  In vivo CRISPR screening directly targeting testicular cells.  Cell Genom  2024    PubMed ID: 38447574   DOI: 10.1016/j.xgen.2024.100510
11170  Nishioka K, Miyazaki H, Soejima H.  Unbiased shRNA screening, using a combination of FACS and high-throughput sequencing, enables identification of novel modifiers of Polycomb silencing.  Sci Rep  2018  8(1):12128  PubMed ID: 30108332   DOI: 10.1038/s41598-018-30649-6
8793  Mezaki Y, Yoshikawa K, Yamaguchi N, Miura M, Imai K, Kato S, Senoo H.  Rat hepatic stellate cells acquire retinoid responsiveness after activation in vitro by post-transcriptional regulation of retinoic acid receptor alpha gene expression.  Arch. Biochem. Biophys.  2007  465:370-9  PubMed ID: 17693386   DOI: 10.1016/

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