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Cell No. : Cell Name
RCB1552 : JHUEM-3  update : 2023/01/04
CommentHuman cell line derived from uterine endometrioid adenocarcinoma. Application consideration
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Contact Us
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.ISHIKAWA Hiroshi (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishikawa, Hiroshi
Originator Ishikawa, H. & Yasuda, M.
Year of deposit 1999
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 60 years
Tissue uterus
Disease name Endometrioid adenocarcinoma,Ib
Classification cancer
Year of origin 1998
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4657
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 0
User's Publication 3

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User's Publication
14047  Yamawaki K, Ishiguro T, Mori Y, Yoshihara K, Suda K, Tamura R, Yamaguchi M, Sekine M, Kashima K, Higuchi M, Fujii M, Okamoto K, Enomoto T.  Sox2-dependent inhibition of p21 is associated with poor prognosis of endometrial cancer  Cancer Sci   2017  108(4):632-640  PubMed ID: 28188685   DOI: 10.1111/cas.13196
14971  Tamura R, Yoshihara K, Yamawaki K, Suda K, Ishiguro T, Adachi S, Okuda S, Inoue I, Verhaak RG, Enomoto T.  Novel kinase fusion transcripts found in endometrial cancer  Sci Rep  2015  5:18657  PubMed ID: 26689674   DOI: 10.1038/srep18657
18132  Weigelt B, Warne PH, Lambros MB, Reis-Filho JS, Downward J.  PI3K pathway dependencies in endometrioid endometrial cancer cell lines  Clin Cancer Res   2013  19(13):3533-44  PubMed ID: 23674493   DOI: 10.1158/1078-0432.CCR-12-3815

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