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Cell No. : Cell Name
RCB1550 : HL-60-R2  update : 2022/04/05
CommentVariant of HL60. Resistant to retinoic acid and active Vit. D derivatives.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Shinshu University
Graduate School of Medicine Institute on Aging and Adaptation
3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan
Dr.SUZUKI Satoru
Fax. +81-263-37-2710
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Suzuki, Satoru
Originator Suzuki, Satoru
Year of deposit 1999
Original cell HL-60
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue blood
Disease name promyelocytic leukemia
Classification cancer
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_4635
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 71-80(50) : /74(13),75(14),76(14),77(2),78(2)/
STR(human) OK
deposit info
lot info
Reference information Reference 3
User's Publication 3

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2408  Mori J, Suzuki S, Hara M, Kaneko A, Yamashita K, Kumagai M, Sakuma T, Kakizawa T, Yamazaki M, Takeda T, Miyamoto T, Ichikawa K, Hashizume K  Characterization of Two Novel Retinoic Acid-resisitant Cell Lines Derived from HL-60 Cells Following Long term Culture with all-trans-Retinoic Acid  Jpn J Cancer Res  1999  90:660-668  PubMed ID: 10429659   DOI: 10.1111/j.1349-7006.1999.tb00798.x
1468  Shimizu N, Ohta M, Fujiwara C, Sagara J, Mochizuki N, Oda T, Utiyama H  A Gene Coding for a Zinc Finger Protein Is Induced during 12-O-Tetradecanoylphorbol-13-Acetate-Stimulated HL-60 Cell Differentiation  J Biochem  1992  111:272-277  PubMed ID: 1569051   DOI: 10.1093/oxfordjournals.jbchem.a123748
973  Collins, S J, Gallo, R C, Gallagher, R E  Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.  Nature  1977  270:347-9  PubMed ID: 271272  

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User's Publication
14523  Yamakawa H, Setoguchi S, Goto S, Watase D, Terada K, Nagata-Akaho N, Toki E, Koga M, Matsunaga K, Karube Y, Takata J.  Growth Inhibitory Effects of Ester Derivatives of Menahydroquinone-4, the Reduced Form of Vitamin K 2(20), on All-Trans Retinoic Acid-Resistant HL60 Cell Line  Pharmaceutics  2021  13(5):758  PubMed ID: 34065416   DOI: 10.3390/pharmaceutics13050758
10054  Sunami Y, Araki M, Kan S, Ito A, Hironaka Y, Imai M, Morishita S, Ohsaka A, Komatsu N.  Histone Acetyltransferase p300/CREB-binding Protein-associated Factor (PCAF) Is Required for All-trans-retinoic Acid-induced Granulocytic Differentiation in Leukemia Cells.  J. Biol. Chem.  2017  292:2815-2829  PubMed ID: 28053092   DOI: 10.1074/jbc.M116.745398
14399  Sakai C, Arai M, Tanaka S, Onda K, Sugiyama K, Hirano T.  Effects of arsenic compounds on growth, cell-cycle distribution and apoptosis of tretinoin-resistant human promyelocytic leukemia cells  Anticancer Res  2014  34(11):6489-94  PubMed ID: 25368250  

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