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Cell No. : Cell Name
RCB1547 : JHUCS-1  update : 2022/07/08
CommentHuman cell line derived from uterine carcinosarcoma. Nude mouse transplantable. Application consideration
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Contact Us
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.ISHIKAWA Hiroshi (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishikawa, Hiroshi
Originator Ishikawa, H. & Yasuda, M. & Yamada, K.
Year of deposit 1999
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 56 years
Tissue uterus
Metastatic ability Yes
Classification cancer
Year of origin 1998
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4652
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 + 15% FBS
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 45-49(50) : 45(1),46(6),47(40),48(2),49(1)
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 3

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3314  Yamada, Kyosuke, Tachibana, Toshiaki, Iida, Yasushi, Ueda, Kazu, Misawa, Akihiko, Suzuki, Nagazumi, Takahashi, Hiroyuki, Kato, Hiroyuki, Kimura, Eizo, Yasuda, Makoto, Tanaka, Tadao, Ishikawa, Hiroshi  Establishment and characterization of JHUCS-1 cell line derived from carcinosarcoma of the human uterus.  Hum Cell  2004  17:139-44  PubMed ID: 15859159   DOI: 10.1111/j.1749-0774.2004.tb00030.x

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User's Publication
19738  Kobayashi Y, Yokoi A, Hashimura M, Oguri Y, Konno R, Matsumoto T, Tochimoto M, Nakagawa M, Ishibashi Y, Ito T, Ohhigata K, Harada Y, Fukagawa N, Kodera Y, Saegusa M.  Nucleobindin-2 Mediates Transforming Growth Factor-β1-Driven Phenotypes in Zinc Finger E-Box Binding Homeobox 1-High Uterine Carcinosarcoma  Am J Pathol  2023  193(8):1116-1128  PubMed ID: 37169340   DOI: 10.1016/j.ajpath.2023.04.011
14686  Tseng JH, Bisogna M, Hoang LN, Olvera N, Rodriguez-Aguayo C, Lopez-Berestein G, Sood AK, Levine DA, Jelinic P.  miR-200c-driven Mesenchymal-To-Epithelial Transition is a Therapeutic Target in Uterine Carcinosarcomas  Sci Rep  2017  7(1):3614  PubMed ID: 28620240   DOI: 10.1038/s41598-017-03972-7
3796  Fukami T, Fukuhara H, Kuramochi M, Maruyama T, Isogai K, Sakamoto M, Takamoto S, Murakami Y.  Promoter methylation of the TSLC1 gene in advanced lung tumors and various cancer cell lines.  Int J Cancer  2003  107(1):53-9  PubMed ID: 12925956   DOI: 10.1002/ijc.11348

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