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Cell No. : Cell Name
RCB1545 : JHUAS-2-L  update : 2022/04/05
CommentHuman B cell-like cell line derived from uterine cancer tissue (Adenosquamous carcinoma).
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.Cell Bank (ISHIKAWA Hiroshi)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishikawa, Hiroshi
Originator Ishikawa, H. & Yasuda, M. & Yamada, K.
Year of deposit 1999
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue B cell (uterus)
Disease name adenosquamous carcinoma
Metastastatic ability Yes
Metastatsd tissue lymph node
Classification transformed
Year of origin 1998
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4651
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM/HamF12 + 15% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 45-46(50) : 45(44),46(6)
STR(human) OK
Reference information Reference 0
User's Publication 0


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