Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB1544 : JHUAS-1  update : 2024/11/20
CommentHuman cell line derived from uterine adenosquamous carcinoma. Said CEA and CA125 producing. Application consideration
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.ISHIKAWA Hiroshi (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishikawa, Hiroshi
Originator Ishikawa, H. & Yamada, K. & Yasuda, M.
Year of deposit 1999
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 56 years
Tissue uterus
Disease name adenosquamous carcinoma
Metastatic ability Yes
Classification cancer
Year of origin 1998
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4650
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 + 15% FBS
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
Chromosome mode 45-47(50) : 45(7),46(42),47(1)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 3


To topTop
Reference

To topTop
User's Publication
18230  Rush CM, Blanchard Z, Polaski JT, Osborne KS, Osby K, Vahrenkamp JM, Yang CH, Lum DH, Hagan CR, Leslie KK, Pufall MA, Thiel KW, Gertz J.  Characterization of HCI-EC-23 a novel estrogen- and progesterone-responsive endometrial cancer cell line  Sci Rep  2022  12(1):19731.  PubMed ID: 36396974   DOI: 10.1038/s41598-022-24211-8
8749  Matsumoto M, Yamaguchi Y, Seino Y, Hatakeyama A, Takei H, Niikura H, Ito K, Suzuki T, Sasano H, Yaegashi N, Hayashi S.  Estrogen signaling ability in human endometrial cancer through the cancer-stromal interaction.  Endocr. Relat. Cancer  2008  15:451-63  PubMed ID: 18508998   DOI: 10.1677/ERC-07-0227
3796  Fukami T, Fukuhara H, Kuramochi M, Maruyama T, Isogai K, Sakamoto M, Takamoto S, Murakami Y.  Promoter methylation of the TSLC1 gene in advanced lung tumors and various cancer cell lines.  Int J Cancer  2003  107(1):53-9  PubMed ID: 12925956   DOI: 10.1002/ijc.11348


Back Back Return Top Page