Cell No. : Cell Name
update : 2024/01/17
Comment Human B cell-like cell line derived from colon cancer tissue.
Comment from the depositor
Terms and conditions A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Contact Us
Order Form
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see ). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp ).
Basic information
Depositor
Ishikawa, Hiroshi
Originator
Ishikawa, H. & Tabei, I. & Kubo, H.
Year of deposit
1999
Animal
_human
< Mammals
Genus
Homo
Species
sapiens
Race
Japanese
Gender
Male
Age at sampling
80 years
Tissue
B cell (colon)
Disease name
adenocarcinoma
Metastatic ability
No
Classification
transformed
Year of origin
1998
Lifespan
infinite
Morphology
lymphocyte-like
Cellosaurus(Expasy)
CVCL_H734
deposit info lot info
Medium
Medium List
Culture type
Suspension cells
Culture method
浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium
DMEM/HamF12 + 20% FBS + 0.1mM NEAA
Antibiotics
Free
Passage method
dilution
Culture information
Passage ratio
1 : 10 split
SC frequency
Subculture : 2 times/week
Temperature
37
℃
CO2 concentration
5
%
Freeze medium
Medium + 10% DMSO
Freezing method
Slow freezing
Mycoplasma/Acholeplasma
(-)
Virus (HIV)
LT 400
Virus (HTLV-1)
(-)
Isozyme
LD, NP
Chromosome mode
90-96(50) : /91(5),92(4).93(13),94(16),95(4)/
STR(human)
OK
Reference information
Reference
0
User's Publication
0
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