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Cell No. : Cell Name
RCB1541 : HFL9t  update : 2022/04/05
CommentHuman fibroblasts derived from fetal lung (19 weekly age). 47,+del(9)(q12). RCB1542 is derived from the same fetus.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.Cell Bank (ISHIKAWA Hiroshi)
Fax. +81-29-836-9130



English
Address
Ishiwata Obstetrics & Gynecology Hospital
1-4-21 Kami-mito, Mito-city 310-0041, Japan
Dr.ISHIWATA Isamu
Fax. +81-29-221-1012
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, I. & Ishikawa, H.
Year of deposit 1999
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 19 weeks embryo
Tissue embryo/fetus, lung
Classification semi-normal
Year of origin 1999
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_B503
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 44-47(50) : 44(1), 45(1), 46(7), 47(41)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
2407  Leichtman, L G, Zackowski, J L, Storto, P D, Newlin A  Non-Mosaic Tetrasomy 9p in a Liveborn Infant With Multiple Congenital Anomalies : Case Report and Comparison With Trisomy 9p  Am J Med Genet  1996  63:434-437  PubMed ID: 8737648  

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