Cell No. : Cell Name
RCB1539 : P19.CL6
update : 2022/04/05
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| Comment | Mouse cell line derived from embryonal carcinoma. C3H strain. Following differentiation, beating cardiomyocytes appear. The efficiency of differentiation is lower compared to RCB 2318. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Murofushi, Kimiko
|
| Originator |
Habara, Akemi
|
| Year of deposit |
1999
|
| Another name |
CL6
|
| Original cell |
P19
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H
|
| Genus |
Mus
|
| Species |
musculus
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonal carcinoma
|
| Classification |
cancer
|
| History |
Originator -> Registor
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_L981
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM ALPHA + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
pipetting or 0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 20 split
|
| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
5
|
| User's Publication |
|
13353
Farooq M, Lindbæk L, Krogh N, Doganli C, Keller C, Mönnich M, Gonçalves AB, Sakthivel S, Mang Y, Fatima A, Andersen VS, Hussain MS, Eiberg H, Hansen L, Kjaer KW, Gopalakrishnan J, Pedersen LB, Møllgård K, Nielsen H, Baig SM, Tommerup N, Christensen ST, Larsen LA.
RRP7A links primary microcephaly to dysfunction of ribosome biogenesis, resorption of primary cilia, and neurogenesis.
Nat Commun
2020
PubMed ID: 33199730
DOI: 10.1038/s41467-020-19658-0
|
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17941
Jin G, Mizutani A, Fukuda T, Otani T, Yan T, Prieto Vila M, Murakami H, Kudoh T, Hirohata S, Kasai T, Salomon DS, Seno M.
Eosinophil cationic protein enhances stabilization of β-catenin during cardiomyocyte differentiation in P19CL6 embryonal carcinoma cells
Mol Biol Rep
2013
40(4):3165-71
PubMed ID: 23271121
DOI: 10.1007/s11033-012-2390-5
|
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18918
Takehara-Kasamatsu Y, Tsuchida K, Nakatani M, Murakami T, Kurisaki A, Hashimoto O, Ohuchi H, Kurose H, Mori K, Kagami S, Noji S, Sugino H.
Characterization of follistatin-related gene as a negative regulatory factor for activin family members during mouse heart development
J Med Invest
2007
54(3-4):276-88
PubMed ID: 17878677
DOI: 10.2152/jmi.54.276
|
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3272
Ohara Y, Atarashi T, Ishibashi T, Ohashi-Kobayashi A, Maeda M.
GATA-4 gene organization and analysis of its promoter.
Biol Pharm Bull
2006
9(3):410-9
PubMed ID: 16508137
DOI: 10.1248/bpb.29.410
|
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5982
Hirata H, Murakami Y, Miyamoto Y, Tosaka M, Inoue K, Nagahashi A, Jakt LM, Asahara T, Iwata H, Sawa Y, Kawamata S.
ALCAM (CD166) is a surface marker for early murine cardiomyocytes.
Cells Tissues Organs
2006
184(3-4):172-80.
PubMed ID: 17409743
DOI: 10.1159/000099624
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