Cell No. : Cell Name RCB1539 : P19.CL6  update : 2022/04/05
Comment			Mouse cell line derived from embryonal carcinoma. C3H strain. Following differentiation, beating cardiomyocytes appear. The efficiency of differentiation is lower compared to RCB 2318.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Murofushi, Kimiko
		Originator	Habara, Akemi
		Year of deposit	1999
		Another name	CL6
		Original cell	P19
		Animal	_mouse < Mammals
		Strain name	C3H
		Genus	Mus
		Species	musculus
		Gender	Male
		Age at sampling	embryo
		Tissue	embryonal carcinoma
		Classification	cancer
		History	Originator -> Registor
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_L981
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM ALPHA + 10% FBS
		Antibiotics		Free
		Passage method		pipetting or 0.25% Trypsin
	Culture information	Passage ratio		1 : 20 split
		SC frequency		Subculture : once/2-3 days
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	4

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Reference

2406  Habara-Ohkubo A.  Differentiation of beating cardiac muscle cells from a derivative of P19 embryonal carcinoma cells.  Cell Struct Funct  1996  21(2):101-10  PubMed ID: 8790939   DOI: 10.1247/csf.21.101

4962  McBurney MW, Rogers BJ.  Isolation of male embryonal carcinoma cells and their chromosome replication patterns.  Dev Biol  1982  89(2):503-8  PubMed ID: 7056443   DOI: 10.1016/0012-1606(82)90338-4

4963  McBurney MW, Jones-Villeneuve EM, Edwards MK, Anderson PJ.  Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line.  Nature  1982  299(5879):165-7  PubMed ID: 7110336   DOI: 10.1038/299165a0

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User's Publication

13353  Farooq M, Lindbæk L, Krogh N, Doganli C, Keller C, Mönnich M, Gonçalves AB, Sakthivel S, Mang Y, Fatima A, Andersen VS, Hussain MS, Eiberg H, Hansen L, Kjaer KW, Gopalakrishnan J, Pedersen LB, Møllgård K, Nielsen H, Baig SM, Tommerup N, Christensen ST, Larsen LA.  RRP7A links primary microcephaly to dysfunction of ribosome biogenesis, resorption of primary cilia, and neurogenesis.  Nat Commun  2020    PubMed ID: 33199730   DOI: 10.1038/s41467-020-19658-0

17941  Jin G, Mizutani A, Fukuda T, Otani T, Yan T, Prieto Vila M, Murakami H, Kudoh T, Hirohata S, Kasai T, Salomon DS, Seno M.  Eosinophil cationic protein enhances stabilization of β-catenin during cardiomyocyte differentiation in P19CL6 embryonal carcinoma cells  Mol Biol Rep  2013  40(4):3165-71  PubMed ID: 23271121   DOI: 10.1007/s11033-012-2390-5

3272  Ohara Y, Atarashi T, Ishibashi T, Ohashi-Kobayashi A, Maeda M.  GATA-4 gene organization and analysis of its promoter.  Biol Pharm Bull  2006  9(3):410-9  PubMed ID: 16508137   DOI: 10.1248/bpb.29.410

5982  Hirata H, Murakami Y, Miyamoto Y, Tosaka M, Inoue K, Nagahashi A, Jakt LM, Asahara T, Iwata H, Sawa Y, Kawamata S.  ALCAM (CD166) is a surface marker for early murine cardiomyocytes.  Cells Tissues Organs  2006  184(3-4):172-80.  PubMed ID: 17409743   DOI: 10.1159/000099624