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Cell No. : Cell Name
RCB1539 : P19.CL6  update : 2022/04/05
CommentMouse cell line derived from embryonal carcinoma. C3H strain. Following differentiation, beating cardiomyocytes appear. The efficiency of differentiation is lower compared to RCB 2318.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Murofushi, Kimiko
Originator Habara, Akemi
Year of deposit 1999
Another name CL6
Original cell P19
Animal mouse < Mammals
Strain name C3H
Genus Mus
Species musculus
Gender Male
Age at sampling embryo
Tissue embryonal carcinoma
Classification cancer
History Originator -> Registor
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_L981
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% Trypsin
Culture information Passage ratio 1 : 20 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 4

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2406  Habara-Ohkubo A.  Differentiation of beating cardiac muscle cells from a derivative of P19 embryonal carcinoma cells.  Cell Struct Funct  1996  21(2):101-10  PubMed ID: 8790939
4962  McBurney MW, Rogers BJ.  Isolation of male embryonal carcinoma cells and their chromosome replication patterns.  Dev Biol  1982  89(2):503-8  PubMed ID: 7056443
4963  McBurney MW, Jones-Villeneuve EM, Edwards MK, Anderson PJ.  Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line.  Nature  1982  299(5879):165-7  PubMed ID: 7110336

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User's Publication
13353  Farooq M, Lindbæk L, Krogh N, Doganli C, Keller C, Mönnich M, Gonçalves AB, Sakthivel S, Mang Y, Fatima A, Andersen VS, Hussain MS, Eiberg H, Hansen L, Kjaer KW, Gopalakrishnan J, Pedersen LB, Møllgård K, Nielsen H, Baig SM, Tommerup N, Christensen ST, Larsen LA.  RRP7A links primary microcephaly to dysfunction of ribosome biogenesis, resorption of primary cilia, and neurogenesis.  Nat Commun  2020    PubMed ID: 33199730
8591  Hirokazu Hirata, Shin Kawamata, Yoshinobu Murakami, Kayoko Inoue, Ayako Nagahashi, Mako Tosaka, Naoko Yoshimura, Yoshiaki Miyamoto, Hiroto Iwasaki, Takayuki Asahara, Yoshiki Sawa  Coexpression of Platelet-Derived Growth Factor Receptor Alpha and Fetal Liver Kinase 1 Enhances Cardiogenic Potential in Embryonic Stem Cell Differentiation in Vitro  J Biosci Bioeng  2007  103 (5), 412-9  PubMed ID: 17609155
3272  Ohara Y, Atarashi T, Ishibashi T, Ohashi-Kobayashi A, Maeda M.  GATA-4 gene organization and analysis of its promoter.  Biol Pharm Bull  2006  9(3):410-9  PubMed ID: 16508137
5982  Hirata H, Murakami Y, Miyamoto Y, Tosaka M, Inoue K, Nagahashi A, Jakt LM, Asahara T, Iwata H, Sawa Y, Kawamata S.  ALCAM (CD166) is a surface marker for early murine cardiomyocytes.  Cells Tissues Organs  2006  184(3-4):172-80.  PubMed ID: 17409743

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