Back Back
Cell No. : Cell Name
RCB1539 : P19.CL6  update : 2022/04/05
CommentMouse cell line derived from embryonal carcinoma. C3H strain. Following differentiation, beating cardiomyocytes appear. The efficiency of differentiation is lower compared to RCB 2318.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Murofushi, Kimiko
Originator Habara, Akemi
Year of deposit 1999
Another name CL6
Original cell P19
Animal _mouse < Mammals
Strain name C3H
Genus Mus
Species musculus
Gender Male
Age at sampling embryo
Tissue embryonal carcinoma
Classification cancer
History Originator -> Registor
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_L981
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% Trypsin
Culture information Passage ratio 1 : 20 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 5

To topTop
2406  Habara-Ohkubo A.  Differentiation of beating cardiac muscle cells from a derivative of P19 embryonal carcinoma cells.  Cell Struct Funct  1996  21(2):101-10  PubMed ID: 8790939   DOI: 10.1247/csf.21.101
4962  McBurney MW, Rogers BJ.  Isolation of male embryonal carcinoma cells and their chromosome replication patterns.  Dev Biol  1982  89(2):503-8  PubMed ID: 7056443   DOI: 10.1016/0012-1606(82)90338-4
4963  McBurney MW, Jones-Villeneuve EM, Edwards MK, Anderson PJ.  Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line.  Nature  1982  299(5879):165-7  PubMed ID: 7110336   DOI: 10.1038/299165a0

To topTop
User's Publication
13353  Farooq M, Lindbæk L, Krogh N, Doganli C, Keller C, Mönnich M, Gonçalves AB, Sakthivel S, Mang Y, Fatima A, Andersen VS, Hussain MS, Eiberg H, Hansen L, Kjaer KW, Gopalakrishnan J, Pedersen LB, Møllgård K, Nielsen H, Baig SM, Tommerup N, Christensen ST, Larsen LA.  RRP7A links primary microcephaly to dysfunction of ribosome biogenesis, resorption of primary cilia, and neurogenesis.  Nat Commun  2020    PubMed ID: 33199730   DOI: 10.1038/s41467-020-19658-0
17941  Jin G, Mizutani A, Fukuda T, Otani T, Yan T, Prieto Vila M, Murakami H, Kudoh T, Hirohata S, Kasai T, Salomon DS, Seno M.  Eosinophil cationic protein enhances stabilization of β-catenin during cardiomyocyte differentiation in P19CL6 embryonal carcinoma cells  Mol Biol Rep  2013  40(4):3165-71  PubMed ID: 23271121   DOI: 10.1007/s11033-012-2390-5
18918  Takehara-Kasamatsu Y, Tsuchida K, Nakatani M, Murakami T, Kurisaki A, Hashimoto O, Ohuchi H, Kurose H, Mori K, Kagami S, Noji S, Sugino H.  Characterization of follistatin-related gene as a negative regulatory factor for activin family members during mouse heart development  J Med Invest  2007  54(3-4):276-88  PubMed ID: 17878677   DOI: 10.2152/jmi.54.276
3272  Ohara Y, Atarashi T, Ishibashi T, Ohashi-Kobayashi A, Maeda M.  GATA-4 gene organization and analysis of its promoter.  Biol Pharm Bull  2006  9(3):410-9  PubMed ID: 16508137   DOI: 10.1248/bpb.29.410
5982  Hirata H, Murakami Y, Miyamoto Y, Tosaka M, Inoue K, Nagahashi A, Jakt LM, Asahara T, Iwata H, Sawa Y, Kawamata S.  ALCAM (CD166) is a surface marker for early murine cardiomyocytes.  Cells Tissues Organs  2006  184(3-4):172-80.  PubMed ID: 17409743   DOI: 10.1159/000099624

Back Back Return Top Page