Cell No. : Cell Name
RCB1538 : 32Dcl3
update : 2024/01/17
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Comment | Subclone of 32D cell line. Differentiate to mature granulocytes by G-CSF. Conditioned medium. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Kamesaki, Hiroshi
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Year of deposit |
1999
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Original cell |
32D
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Animal |
_mouse
< Mammals
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Strain name |
C3H/HeJ
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Tissue |
bone marrow
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Disease name |
myeloid precursor cell
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Classification |
transformed
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Lifespan |
infinite
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Morphology |
lymphocyte-like
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Cellosaurus(Expasy) |
CVCL_0119
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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Culture medium |
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IMDM (4mM L-Glutamine) + 10% FBS + 10% (RCB0035 WEHI-3 conditioned medium)
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Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 5 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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SSLP(mouse) |
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OK
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Isozyme |
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LD, NP
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Images |
deposit info | lot info |
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Reference information |
Reference |
9
|
User's Publication |
5
|
Reference |
5470
Patel G, Kreider B, Rovera G, Reddy EP.
v-myb blocks granulocyte colony-stimulating factor-induced myeloid cell differentiation but not proliferation.
Mol Cell Biol
1993
13(4):2269-76
PubMed ID: 7681145
DOI: 10.1128/mcb.13.4.2269
|
2259
Kreider BL, Benezra R, Rovera G, Kadesch T.
Inhibition of myeloid differentiation by the helix-loop-helix protein Id.
Science
1992
255(5052):1700-2
PubMed ID: 1372755
DOI: 10.1126/science.1372755
|
5468
Friedman AD, Krieder BL, Venturelli D, Rovera G.
Transcriptional regulation of two myeloid-specific genes, myeloperoxidase and lactoferrin, during differentiation of the murine cell line 32D C13.
Blood
1991
78(9):2426-32
PubMed ID: 1718497
|
2260
Kreider BL, Phillips PD, Prystowsky MB, Shirsat N, Pierce JH, Tushinski R, Rovera G.
Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell.
Mol Cell Biol
1990
10(9):4846-53
PubMed ID: 1697033
DOI: 10.1128/mcb.10.9.4846
|
5466
Migliaccio G, Migliaccio AR, Kreider BL, Rovera G, Adamson JW.
Selection of lineage-restricted cell lines immortalized at different stages of hematopoietic differentiation from the murine cell line 32D.
J Cell Biol
1989
109(2):833-41
PubMed ID: 2668305
DOI: 10.1083/jcb.109.2.833
|
4924
Tweardy DJ1, Caracciolo D, Valtieri M, Rovera G.
Tumor-derived growth factors that support proliferation and differentiation of normal and leukemic hemopoietic cells.
Ann N Y Acad Sci
1987
511:30-8
PubMed ID: 2830826
DOI: 10.1111/j.1749-6632.1987.tb36235.x
|
4925
Rovera G, Valtieri M, Mavilio F, Reddy EP.
Effect of Abelson murine leukemia virus on granulocytic differentiation and interleukin-3 dependence of a murine progenitor cell line.
Oncogene
1987
1(1):29-35
PubMed ID: 2449644
|
2770
Valtieri M, Tweardy DJ, Caracciolo D, Johnson K, Mavilio F, Altmann S, Santoli D, Rovera G.
Cytokine-dependent granulocytic differentiation. Regulation of proliferative and differentiative responses in a murine progenitor cell line.
J Immunol
1987
138(11):3829-35
PubMed ID: 2438328
|
1979
Greenberger, J S, Sakakeeny, M A, Humphries, R K, Eaves, C J, Eckner, R J
Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines.
Proc Natl Acad Sci U S A
1983
80:2931-5
PubMed ID: 6574462
DOI: 10.1073/pnas.80.10.2931
|
User's Publication |
4869
Kitagawa J, Hara T, Tsurumi H, Kanemura N, Kasahara S, Shimizu M, Moriwaki H.
Cell cycle-dependent priming action of granulocyte colony-stimulating factor (G-CSF) enhances in vitro apoptosis induction by cytarabine and etoposide in leukemia cell lines.
J Clin Exp Hematop
2010
50(2):99-105
PubMed ID: 21123967
DOI: 10.3960/jslrt.50.99
|
18476
Aoki N, Kimura Y, Kimura S, Nagato T, Azumi M, Kobayashi H, Sato K, Tateno M.
Expression and functional role of MDL-1 (CLEC5A) in mouse myeloid lineage cells
J Leukoc Biol
2009
85(3):508-17
PubMed ID: 19074552
DOI: 10.1189/jlb.0508329
|
18167
Jun-ichi Kitagawa, Takeshi Hara, Hisashi Tsurumi, Nobuhiro Kanemura, Masahito Shimizu, Hisataka Moriwai
Priming Effect with G-CSF Enhances In Vitro Apoptosis by Treatment with Ara-C and VP-16 in Leukemic Cell Line
Blood
2007
110:4333-4333
DOI: 10.1182/blood.v110.11.4333.4333
|
18674
Yamaguchi M, Fujimura K, Toga H, Khwaja A, Okamura N, Chopra R.
Shwachman-Diamond syndrome is not necessary for the terminal maturation of neutrophils but is important for maintaining viability of granulocyte precursors
Exp Hematol
2007
35(4):579-86
PubMed ID: 17379068
DOI: 10.1016/j.exphem.2006.12.010
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4892
Kumagai H1, Oki T, Tamitsu K, Feng SZ, Ono M, Nakajima H, Bao YC, Kawakami Y, Nagayoshi K, Copeland NG, Gilbert DJ, Jenkins NA, Kawakami T, Kitamura T.
Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells.
Biochem Biophys Res Commun
2003
307(3):719-29
PubMed ID: 12893283
DOI: 10.1016/s0006-291x(03)01245-2
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