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Cell No. : Cell Name
RCB1538 : 32Dcl3  update : 2022/07/20
CommentSubclone of 32D cell line. Differentiate to mature granulocytes by G-CSF. Conditioned medium.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kamesaki, Hiroshi
Year of deposit 1999
Original cell 32D
Animal mouse < Mammals
Strain name C3H/HeJ
Tissue bone marrow
Disease name myeloid precursor cell
Classification transformed
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0119
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives IMDM (4mM L-Glutamine) + 10% FBS + 10% (RCB0035 WEHI-3 conditioned medium)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 9
User's Publication 2

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5470  Patel G, Kreider B, Rovera G, Reddy EP.  v-myb blocks granulocyte colony-stimulating factor-induced myeloid cell differentiation but not proliferation.  Mol Cell Biol  1993  13(4):2269-76  PubMed ID: 7681145
2259  Kreider BL, Benezra R, Rovera G, Kadesch T.  Inhibition of myeloid differentiation by the helix-loop-helix protein Id.  Science  1992  255(5052):1700-2  PubMed ID: 1372755
5468  Friedman AD, Krieder BL, Venturelli D, Rovera G.  Transcriptional regulation of two myeloid-specific genes, myeloperoxidase and lactoferrin, during differentiation of the murine cell line 32D C13.  Blood  1991  78(9):2426-32  PubMed ID: 1718497
2260  Kreider BL, Phillips PD, Prystowsky MB, Shirsat N, Pierce JH, Tushinski R, Rovera G.  Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell.  Mol Cell Biol  1990  10(9):4846-53  PubMed ID: 1697033
5466  Migliaccio G, Migliaccio AR, Kreider BL, Rovera G, Adamson JW.  Selection of lineage-restricted cell lines immortalized at different stages of hematopoietic differentiation from the murine cell line 32D.  J Cell Biol  1989  109(2):833-41  PubMed ID: 2668305
4924  Tweardy DJ1, Caracciolo D, Valtieri M, Rovera G.  Tumor-derived growth factors that support proliferation and differentiation of normal and leukemic hemopoietic cells.  Ann N Y Acad Sci  1987  511:30-8  PubMed ID: 2830826
4925  Rovera G, Valtieri M, Mavilio F, Reddy EP.  Effect of Abelson murine leukemia virus on granulocytic differentiation and interleukin-3 dependence of a murine progenitor cell line.  Oncogene  1987  1(1):29-35  PubMed ID: 2449644
2770  Valtieri M, Tweardy DJ, Caracciolo D, Johnson K, Mavilio F, Altmann S, Santoli D, Rovera G.  Cytokine-dependent granulocytic differentiation. Regulation of proliferative and differentiative responses in a murine progenitor cell line.  J Immunol  1987  138(11):3829-35  PubMed ID: 2438328
1979  Greenberger, J S, Sakakeeny, M A, Humphries, R K, Eaves, C J, Eckner, R J  Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines.  Proc Natl Acad Sci U S A  1983  80:2931-5  PubMed ID: 6574462

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User's Publication
4869  Kitagawa J, Hara T, Tsurumi H, Kanemura N, Kasahara S, Shimizu M, Moriwaki H.  Cell cycle-dependent priming action of granulocyte colony-stimulating factor (G-CSF) enhances in vitro apoptosis induction by cytarabine and etoposide in leukemia cell lines.  J Clin Exp Hematop  2010  50(2):99-105  PubMed ID: 21123967
4892  Kumagai H1, Oki T, Tamitsu K, Feng SZ, Ono M, Nakajima H, Bao YC, Kawakami Y, Nagayoshi K, Copeland NG, Gilbert DJ, Jenkins NA, Kawakami T, Kitamura T.  Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells.  Biochem Biophys Res Commun  2003  307(3):719-29  PubMed ID: 12893283

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