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Cell No. : Cell Name
RCB1537 : MLC-7  update : 2022/04/05
CommentBone marrow stroma cell line, depositing calcified matrix on the culture surface.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sakiyama, Hisako
Originator Sakiyama, Hisako
Year of deposit 1999
Animal mouse < Mammals
Strain name C3H/He
Tissue bone marrow
Classification semi-normal
Year of origin 1994
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_2I00
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives McCoy's 5A + 20% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage cell No 1 x 10^(6) cells/60 mm dish
SC frequency Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, PepB
Reference information Reference 0
User's Publication 2


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Reference

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User's Publication
7863  Maehira F1, Iinuma Y, Eguchi Y, Miyagi I, Teruya S.  Effects of soluble silicon compound and deep-sea water on biochemical and mechanical properties of bone and the related gene expression in mice.  J Bone Miner Metab  2008  26(5):446-55  PubMed ID: 18758902   DOI: 10.1007/s00774-007-0845-x
2771  Sakiyama H, Masuda R, Inoue N, Yamamoto K, Kuriiwa K, Nakagawa K, Yoshida K  Establishment and characterization of macrophage-like cell lines expressing osteoclast-specific markers.  J Bone Miner Metab  2001  19:220-7  PubMed ID: 11448014   DOI: 10.1007/s007740170024



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