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Cell No. : Cell Name
RCB1536 : MLC-6  update : 2022/04/05
CommentOsteoclast-like cell line derived from mouse (C3H/He).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sakiyama, Hisako
Originator Sakiyama, Hisako
Year of deposit 1999
Animal mouse < Mammals
Strain name C3H/He
Tissue bone marrow
Classification semi-normal
Year of origin 1994
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_2H99
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives McCoy's 5A + 20% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 0
User's Publication 4

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User's Publication
9378  R Toita, Sunarso, A N Rashid, K Tsuru, K Ishikawa  Modulation of the Osteoconductive Property and Immune Response of Poly(ether Ether Ketone) by Modification With Calcium Ions  J Mater Chem B  2015  3(44):8738-8746  PubMed ID: 32262731   DOI: 10.1039/c5tb01679g
8321  Yamashita M, Otsuka F, Mukai T, Yamanaka R, Otani H, Matsumoto Y, Nakamura E, Takano M, Sada KE, Makino H.  Simvastatin inhibits osteoclast differentiation induced by bone morphogenetic protein-2 and RANKL through regulating MAPK, AKT and Src signaling.  Regul. Pept.  2010  162:99-108  PubMed ID: 20346376   DOI: 10.1016/j.regpep.2010.03.003
8584  Tanaka T, Kawashima H, Ohnishi K, Matsumura K, Yoshimura R, Matsuyama M, Kuratsukuri K, Nakatani T.  Indirect antitumor effects of bisphosphonates on prostatic LNCaP cells co-cultured with bone cells.  Anticancer Res.  2009  29:1089-94  PubMed ID: 19414350  
2771  Sakiyama H, Masuda R, Inoue N, Yamamoto K, Kuriiwa K, Nakagawa K, Yoshida K  Establishment and characterization of macrophage-like cell lines expressing osteoclast-specific markers.  J Bone Miner Metab  2001  19:220-7  PubMed ID: 11448014   DOI: 10.1007/s007740170024

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