Cell No. : Cell Name
RCB1536 : MLC-6
update : 2023/08/23
|
| Comment | Osteoclast-like cell line derived from mouse (C3H/He). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Sakiyama, Hisako
|
| Originator |
Sakiyama, Hisako
|
| Year of deposit |
1999
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H/He
|
| Tissue |
bone marrow
|
| Classification |
semi-normal
|
| Year of origin |
1994
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_2H99
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
McCoy's 5A + 20% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
pipetting
|
Culture information
|
Passage ratio |
|
1 : 4-8 split
|
| SC frequency |
|
Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
0
|
| User's Publication |
4
|
| User's Publication |
|
9378
R Toita, Sunarso, A N Rashid, K Tsuru, K Ishikawa
Modulation of the Osteoconductive Property and Immune Response of Poly(ether Ether Ketone) by Modification With Calcium Ions
J Mater Chem B
2015
3(44):8738-8746
PubMed ID: 32262731
DOI: 10.1039/c5tb01679g
|
|
8321
Yamashita M, Otsuka F, Mukai T, Yamanaka R, Otani H, Matsumoto Y, Nakamura E, Takano M, Sada KE, Makino H.
Simvastatin inhibits osteoclast differentiation induced by bone morphogenetic protein-2 and RANKL through regulating MAPK, AKT and Src signaling.
Regul. Pept.
2010
162:99-108
PubMed ID: 20346376
DOI: 10.1016/j.regpep.2010.03.003
|
|
8584
Tanaka T, Kawashima H, Ohnishi K, Matsumura K, Yoshimura R, Matsuyama M, Kuratsukuri K, Nakatani T.
Indirect antitumor effects of bisphosphonates on prostatic LNCaP cells co-cultured with bone cells.
Anticancer Res.
2009
29:1089-94
PubMed ID: 19414350
|
|
2771
Sakiyama H, Masuda R, Inoue N, Yamamoto K, Kuriiwa K, Nakagawa K, Yoshida K
Establishment and characterization of macrophage-like cell lines expressing osteoclast-specific markers.
J Bone Miner Metab
2001
19:220-7
PubMed ID: 11448014
DOI: 10.1007/s007740170024
|
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