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Cell No. : Cell Name
RCB1528 : CCP-2  update : 2022/04/05
CommentBone marrow stroma cell line, depositing calcified matrix on the culture surface. TRAP-positive.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sakiyama, Hisako
Originator Sakiyama, Hisako
Year of deposit 1999
Animal hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Tissue bone marrow
Classification semi-normal
Year of origin 1994
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4566
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives McCoy's 5A + 20% FBS
Antibiotics Free
Passage method pipetting (softly)
Culture information Passage cell No 1 x 10^(6) cells/60 mm dish
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
Reference information Reference 0
User's Publication 2


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Reference

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User's Publication
2773  Sakiyama H, Nonaka T, Masuda R, Inoue N, Kuboki Y, Iijima M, Hirabayasi Y, Takahagi M, Yoshida K, Kuriiwa K, Yoshida M, Imajoh-Ohmi S  Characterization of mineral deposits formed in cultures of a hamster tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) double-positive cell line (CCP).  Cell Tissue Res  2002  309:269-79  PubMed ID: 12172786
2772  Masuda R, Sakiyama H, Nonaka T, Kwan A, Nakagawa K, Moriya H, Imajoh-Ohmi S, Honjo M, Yoshida K  Establishment and characterization of tartrate-resistant acid phosphatase and alkaline phosphatase double positive cell lines.  Cell Tissue Res  2001  304:351-9  PubMed ID: 11456411



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