Cell No. : Cell Name
RCB1525 : HeLa.S3
update : 2023/06/30
|
| Comment | RCB0191 HeLa.S3, that has been cultured in MEM(susp.). This line is cultured in MEM. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ohno, Tadao
|
| Year of deposit |
1999
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_0058
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
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Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
65-70(50) : 65(4),66(10),67(19),68(7),69(6),70(4)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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|
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| Reference information |
Reference |
5
|
| User's Publication |
7
|
| Reference |
|
350
Kuroki T.
Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.
Exp Cell Res
1973
80(1):55-62
PubMed ID: 4361348
DOI: 10.1016/0014-4827(73)90274-7
|
|
1446
J E DARNELL Jr, T K SAWYERc
Variation in plaque-forming ability among parental and clonal strains of HeLa cells
Virology
1959
223-9
PubMed ID: 13669339
DOI: 10.1016/0042-6822(59)90006-6
|
|
1445
SATO G, FISHER HW, PUCK TT.
Molecular growth requirements of single mammalian cells.
Science
1957
126(3280):461-4
PubMed ID: 13486039
|
|
206
PUCK TT, MARCUS PI, CIECIURA SJ.
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
J Exp Med
1956
103(2):273-83
PubMed ID: 13286432
DOI: 10.1084/jem.103.2.273
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
| User's Publication |
|
22118
Inagaki M, Abe N, Li Z, Nakashima Y, Acharyya S, Ogawa K, Kawaguchi D, Hiraoka H, Banno A, Meng Z, Tada M, Ishida T, Lyu P, Kokubo K, Murase H, Hashiya F, Kimura Y, Uchida S, Abe H.
Cap analogs with a hydrophobic photocleavable tag enable facile purification of fully capped mRNA with various cap structures.
Nat Commun
2024
14(1):2657
PubMed ID: 37169757
DOI: 10.1038/s41467-023-38244-8
|
|
17789
Takano S, Shibamoto Y, Wang Z, Kondo T, Hashimoto S, Kawai T, Hiwatashi A.
Optimal timing of a γH2AX analysis to predict cellular lethal damage in cultured tumor cell lines after exposure to diagnostic and therapeutic radiation doses
J Radiat Res
2023
64(2):317-327
PubMed ID: 36634337
DOI: 10.1093/jrr/rrac096
|
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19557
Saito H, Osaki T, Ikeuchi Y, Iwasaki S.
High-throughput Assessment of Mitochondrial Protein Synthesis in Mammalian Cells Using Mito-FUNCAT FACS
Bio Protoc
2023
13(3):e4602
PubMed ID: 36816992
DOI: 10.21769/BioProtoc.4602
|
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20330
Chhipi-Shrestha JK, Schneider-Poetsch T, Suzuki T, Mito M, Khan K, Dohmae N, Iwasaki S, Yoshida M.
Splicing modulators elicit global translational repression by condensate-prone proteins translated from introns.
Cell Chem Biol
2022
PubMed ID: 34520743
DOI: 10.1016/j.chembiol.2021.07.015
|
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20596
Kimura Y, Saito H, Osaki T, Ikegami Y, Wakigawa T, Ikeuchi Y, Iwasaki S.
Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation.
RNA
2022
PubMed ID: 35256452
DOI: 10.1261/rna.079097.122
|
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20819
Chhipi-Shrestha JK, Yoshida M, Iwasaki S.
Filter trapping protocol to detect aggregated proteins in human cell lines.
STAR Protoc
2022
3(3)
PubMed ID: 35880124
DOI: 10.1016/j.xpro.2022.101571
|
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15562
Yasumitsu Ogra, Saki Onishi, Asami Kajiwara, Atsuko Hara, Kazuo T. Suzuki
Enhancement of Nuclear Localization of Metallothionein by Nitric Oxide
Journal of Health Science
2008
54:339-342
DOI: 10.1248/jhs.54.339
|
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