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Cell No. : Cell Name
RCB1525 : HeLa.S3  update : 2023/06/30
CommentRCB0191 HeLa.S3, that has been cultured in MEM(susp.). This line is cultured in MEM.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Year of deposit 1999
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0058
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 65-70(50) : 65(4),66(10),67(19),68(7),69(6),70(4)
STR(human) OK
deposit info
lot info
Reference information Reference 5
User's Publication 6

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350  Kuroki T.  Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.  Exp Cell Res  1973  80(1):55-62  PubMed ID: 4361348   DOI: 10.1016/0014-4827(73)90274-7
1446  J E DARNELL Jr, T K SAWYERc  Variation in plaque-forming ability among parental and clonal strains of HeLa cells  Virology  1959  223-9  PubMed ID: 13669339   DOI: 10.1016/0042-6822(59)90006-6
1445  SATO G, FISHER HW, PUCK TT.  Molecular growth requirements of single mammalian cells.  Science  1957  126(3280):461-4  PubMed ID: 13486039  
206  PUCK TT, MARCUS PI, CIECIURA SJ.  Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.  J Exp Med  1956  103(2):273-83  PubMed ID: 13286432   DOI: 10.1084/jem.103.2.273
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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User's Publication
17789  Takano S, Shibamoto Y, Wang Z, Kondo T, Hashimoto S, Kawai T, Hiwatashi A.  Optimal timing of a γH2AX analysis to predict cellular lethal damage in cultured tumor cell lines after exposure to diagnostic and therapeutic radiation doses  J Radiat Res  2023  64(2):317-327  PubMed ID: 36634337   DOI: 10.1093/jrr/rrac096
19557  Saito H, Osaki T, Ikeuchi Y, Iwasaki S.  High-throughput Assessment of Mitochondrial Protein Synthesis in Mammalian Cells Using Mito-FUNCAT FACS  Bio Protoc  2023  13(3):e4602  PubMed ID: 36816992   DOI: 10.21769/BioProtoc.4602
20330  Chhipi-Shrestha JK, Schneider-Poetsch T, Suzuki T, Mito M, Khan K, Dohmae N, Iwasaki S, Yoshida M.  Splicing modulators elicit global translational repression by condensate-prone proteins translated from introns.  Cell Chem Biol  2022    PubMed ID: 34520743   DOI: 10.1016/j.chembiol.2021.07.015
20596  Kimura Y, Saito H, Osaki T, Ikegami Y, Wakigawa T, Ikeuchi Y, Iwasaki S.  Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation.  RNA  2022    PubMed ID: 35256452   DOI: 10.1261/rna.079097.122
20819  Chhipi-Shrestha JK, Yoshida M, Iwasaki S.  Filter trapping protocol to detect aggregated proteins in human cell lines.  STAR Protoc  2022  3(3)  PubMed ID: 35880124   DOI: 10.1016/j.xpro.2022.101571
15562  Yasumitsu Ogra, Saki Onishi, Asami Kajiwara, Atsuko Hara, Kazuo T. Suzuki  Enhancement of Nuclear Localization of Metallothionein by Nitric Oxide  Journal of Health Science  2008  54:339-342    DOI: 10.1248/jhs.54.339

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