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Cell No. : Cell Name
RCB1518 : SS-A3-1  update : 2022/04/04
CommentF344 male rat fibrosarcoma cells, but with histiocytic and/or myofibroblastic characteristics.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Yamate, Jyoji
Originator Yamate, Jyoji
Year of deposit 1999
Original cell SS-P
Animal rat < Mammals
Strain name F344/DuCrj
Gender Male
Tissue submandibular salivary gland
Disease name fibrosarcoma
Classification semi-normal
Year of origin 1995
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5367
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
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lot info
Reference information Reference 2
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2404  Yamate J, Iwaki M, Nakatsuji S, Kuwamura M, Kotani T, Sakuma S  Lysozyme-containing renal tubular hyaline droplets in F344 rats bearing a rat fibrosarcoma-derived transplantable tumor.  Toxicol Pathol  1998  26:699-703  PubMed ID: 9789960
2403  Yamate J, Iwaki M, Kumagai D, Tsukamoto Y, Kuwamura M, Nakatsuji S, Tsunenari I, Kotani T, Sakuma S  Characteristics of a rat fibrosarcoma-derived transplantable tumour line (SS) and cultured cell lines (SS-P and SS-A3-1) showing myofibroblastic and histiocytic phenotypes.  Virchows Arch  1997  431:431-40  PubMed ID: 9428931

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