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Cell No. : Cell Name
RCB1513 : HOMM  update : 2022/04/04
CommentHuman cell line derived from melanoma occurred in uterine cervix. Possessing melanin granules.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, I. & Kiguchi, K. & Ishikawa, H.
Year of deposit 1999
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 65 years
Tissue cervix
Disease name malignant melanoma
Classification cancer
Year of origin 1996
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4842
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 118-139(50) : /125(5),126(3),127(5),128(4),129(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 1

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2402  Kiguchi K, Ishiwata I, Ishiwata C, Tokieda Y, Iguchi M, Suzuki R, Saga M, Ishikawa H  Establishment and characterization of melanoma cell line derived from malignant melanoma of human uterine cervix.  Hum Cell  1998  11:93-100  PubMed ID: 9777403  

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User's Publication
11645  Yamada H, Hakozaki M, Uemura A, Yamashita T.  Effect of fatty acids on melanogenesis and tumor cell growth in melanoma cells.  J. Lipid Res.  2019    PubMed ID: 31345992   DOI: 10.1194/jlr.M090712

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