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Cell No. : Cell Name
RCB1512 : MH7A  update : 2021/02/26
CommentRheumatoid fibroblast-like synoviocyte transformed with SV40 T antigen. Respond to IL-1beta.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Kissei Pharmaceutical Co., Ltd.

4365-1 Kashihara, Hodaka, Azumino, Nagano 399-8304 Japan
Dr.R & D, Planning
Fax. +81-263-82-8826
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor R & D, Planning
Originator Miyazawa, Keiji
Year of deposit 1999
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 53 years
Tissue synovial
Disease name Rheumatoid Arthritis (RA)
Classification transformed
Recombinant recombinant
Exogene SV40 large T antigen
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 66-82(50) : /73(6),74(4),75(6),76(3),77(7),78(5)/
deposit info
lot info
Reference information Reference 2
User's Publication 45

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2339  Miyazawa K, Mori A, Yamamoto K, Okudaira H  Transcriptional roles of CCAAT/enhancer binding protein-beta, nuclear factor-kappaB, and C-promoter binding factor 1 in interleukin (IL)-1beta-induced IL-6 synthesis by human rheumatoid fibroblast-like synoviocytes.  J Biol Chem  1998  273:7620-7  PubMed ID: 9516466
2338  Miyazawa K, Mori A, Okudaira H  Establishment and Characterization of a Novel Human Rheumatoid Fibroblast-Like Synoviocyte Line, MH7A, Immortalized with SV40 T Antigen  J Biochem  1998  124:1153-1162  PubMed ID: 9832620

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User's Publication
13169  Fu X, Song G, Ni R, Liu H, Xu Z, Zhang D, He F, Huang G.  LncRNA-H19 silencing suppresses synoviocytes proliferation and attenuates collagen-induced arthritis progression by modulating miR-124a.  Rheumatology (Oxford)  2021    PubMed ID: 32810279
13891  Kenta Kaihara, Shuji Nakagawa, Yuji Arai, Hiroaki Inoue, Shinji Tsuchida, Yuta Fujii, Yoichiro Kamada, Tsunao Kishida, Osam Mazda, Kenji Takahashi  Sustained Hypoxia Suppresses Joint Destruction in a Rat Model of Rheumatoid Arthritis via Negative Feedback of Hypoxia Inducible Factor-1α  International Journal of Molecular Sciences  2021  22:3898 
12156  Xiangbo Meng, Wenxiang Cheng, Shan Zhong, Peng Zhang, Ling Qin & Xinluan Wang  Anti-inflammatory effects of Jingshu Keli capsule and its components on human synoviocyte MH7A cells  Arthroplasty  2020   
13031  Yamagishi Y, Someya A, Nagaoka I.  Citrulline cooperatively exerts an anti-inflammatory effect on synovial cells with glucosamine and N-acetylglucosamine.  Biomed Rep  2020    PubMed ID: 32440348
13058  Tsai MH, Chi MC, Hsu JF, Lee IT, Lin KM, Fang ML, Lee MH, Lee CW, Liu JF.  Urban Particulate Matter Enhances ROS/IL-6/COX-II Production by Inhibiting MicroRNA-137 in Synovial Fibroblast of Rheumatoid Arthritis.  Cells  2020    PubMed ID: 32498294
13149  Lee J, Yoon SS, Thuy PX, Moon EY.  Synovial Cell Migration is Associated with B Cell Activating Factor Expression Increased by TNFα or Decreased by KR33426.  Biomol Ther (Seoul)  2020    PubMed ID: 32753567
13503  Wu H, He Y, Wu H, Zhou M, Xu Z, Xiong R, Yan F, Liu H.  Near-infrared fluorescence imaging-guided focused ultrasound-mediated therapy against Rheumatoid Arthritis by MTX-ICG-loaded iRGD-modified echogenic liposomes  Theranostics  2020  10(22):10092-10105  PubMed ID: 32929336
13519  Wu S, Wang J, Li J, Li F.  microRNA-21 Aggravates Lipopolysaccharide-Induced Inflammation in MH7A Cells Through Targeting SNF5  Inflammation  2020  43(2):441-454  PubMed ID: 32008163
13540  Kim J, Jung KH, Yoo J, Park JH, Yan HH, Fang Z, Lim JH, Kwon SR, Kim MK, Park HJ, Hong SS.  PBT-6, a Novel PI3KC2γ Inhibitor in Rheumatoid Arthritis  Biomol Ther (Seoul)  2020  28(2):172-183  PubMed ID: 31739383
13563  Wang T, Jia Q, Chen T, Yin H, Tian X, Lin X, Liu Y, Zhao Y, Wang Y, Shi Q, Huang C, Xu H, Liang Q.  Alleviation of Synovial Inflammation of Juanbi-Tang on Collagen-Induced Arthritis and TNF-Tg Mice Model  Front Pharmacol  2020  11:45  PubMed ID: 32116720
13644  Hsiang-Ping Lee, Yang-Chang Wu, Bo-Cheng Chen, Shan-Chi Liu, Te-Mao Li, Wei-Chien Huang, Chin-Jung Hsu, Chih-Hsin Tang  Soya-cerebroside reduces interleukin production in human rheumatoid arthritis synovial fibroblasts by inhibiting the ERK, NF-κB and AP-1 signalling pathways  Food and Agricultural Immunology  2020  21(740-750) 
13645  Kanai T, Kondo N, Okada M, Sano H, Okumura G, Kijima Y, Ogose A, Kawashima H, Endo N.  The JNK pathway represents a novel target in the treatment of rheumatoid arthritis through the suppression of MMP-3  J Orthop Surg Res  2020  15(1):87  PubMed ID: 32131874
11298  Ma J, Wang X, Lv T, Liu J, Ren Y, Zhang J, Zhang Y.  Effects of Ghrelin on the Apoptosis of Rheumatoid Arthritis Fibroblast-Like Synoviocyte MH7A Cells.  Biol. Pharm. Bull.  2019    PubMed ID: 30429411
11434  Hayakawa K, Kawasaki M, Hirai T, Yoshida Y, Tsushima H, Fujishiro M, Ikeda K, Morimoto S, Takamori K, Sekigawa I.  MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling.  Int J Mol Sci  2019    PubMed ID: 30769772
11529  Meng D, Li J, Li H, Wang K.  Salvianolic acid B remits LPS-induced injury by up-regulating miR-142-3p in MH7A cells.  Biomed. Pharmacother.  2019    PubMed ID: 31022596
11688  Wen-Xiang Cheng, Huan Huang, Jian-Hai Chen, Tian-Tian Zhang, Guo-Yuan Zhu, Zheng-Tan Zheng, Jie-Tao Lin, Yi-Ping Hu, Yong Zhang, Xue-Ling Bai, Yan Wang, Zhan-Wang Xu, Bing Song, Yi-Ying Mao, Fei Yang, Peng Zhang  Genistein inhibits angiogenesis developed during rheumatoid arthritis through the IL-6/JAK2/STAT3/VEGF signalling pathway  Journal of Orthopaedic Translation  2019    PubMed ID: 32440504
12330  Jia Q, Wang T, Wang X, Xu H, Liu Y, Wang Y, Shi Q, Liang Q.  Astragalin Suppresses Inflammatory Responses and Bone Destruction in Mice With Collagen-Induced Arthritis and in Human Fibroblast-Like Synoviocytes  Front Pharmacol  2019  10:94  PubMed ID: 30809149
12519  Chen H, Liang Q, Zhou X, Wang X.  Preparative separation of the flavonoid fractions from Periploca forrestii Schltr. ethanol extracts using macroporous resin combined with HPLC analysis and evaluation of their biological activities  J Sep Sci  2019  42(3):650-661  PubMed ID: 30461196
12520  Huang CC, Chiou CH, Liu SC, Hu SL, Su CM, Tsai CH, Tang CH.  Melatonin attenuates TNF-α and IL-1β expression in synovial fibroblasts and diminishes cartilage degradation: Implications for the treatment of rheumatoid arthritis  J Pineal Res  2019  66(3):e12560  PubMed ID: 30648758
10840  Jia W, Wu W, Yang D, Xiao C, Huang M, Long F, Su Z, Qin M, Liu X, Zhu YZ.  GATA4 regulates angiogenesis and persistence of inflammation in rheumatoid arthritis.  Cell Death Dis  2018  9:503  PubMed ID: 29717129
11091  Takeno M, Kitagawa S, Yamanaka J, Teramoto M, Tomita H, Shirai N, Itoh S, Hida S, Hayakawa K, Onozaki K, Takii T.  5-Hydroxy-2-methylpyridine Isolated from Cigarette Smoke Condensate Aggravates Collagen-Induced Arthritis in Mice.  Biol. Pharm. Bull.  2018  41(6):877-884  PubMed ID: 29863076
11169  Wang X, Wang X, Sun J, Fu S.  An enhanced RRM2 siRNA delivery to rheumatoid arthritis fibroblast-like synoviocytes through a liposome-protamine-DNA-siRNA complex with cell permeable peptides.  Int. J. Mol. Med.  2018  42(5):2393-2402  PubMed ID: 30106091
11203  Zhong F, Xu J, Yang X, Zhang Q, Gao Z, Deng Y, Zhang L, Yu C.  miR-145 eliminates lipopolysaccharides-induced inflammatory injury in human fibroblast-like synoviocyte MH7A cells.  J. Cell. Biochem.  2018    PubMed ID: 30191608
4296  He L, Liu C, Sun C, Wang J, Zhi K, Sun D, Wang H, Wang Q, Lin N.  Wu-Tou Decoction Inhibits Angiogenesis in Experimental Arthritis by Targeting VEGFR2 Signaling Pathway.  Rejuvenation Res  2018    PubMed ID: 29385909
4297  Wu J, Fan W, Ma L, Geng X.  miR-708-5p promotes fibroblast-like synoviocytes' cell apoptosis and ameliorates rheumatoid arthritis by the inhibition of Wnt3a/β-catenin pathway.  Drug Des Devel Ther  2018    PubMed ID: 30349197
10252  Lee JW, Lee J, Um SH, Moon EY.  Synovial cell death is regulated by TNF-α-induced expression of B-cell activating factor through an ERK-dependent increase in hypoxia-inducible factor-1α.  Cell Death Dis  2017  8:e2727  PubMed ID: 28383556
10404  Tsushima H, Morimoto S, Fujishiro M, Yoshida Y, Hayakawa K, Hirai T, Miyashita T, Ikeda K, Yamaji K, Takamori K, Takasaki Y, Sekigawa I, Tamura N.  Kinase inhibitors of the IGF-1R as a potential therapeutic agent for rheumatoid arthritis.  Autoimmunity  2017  50:329-335  PubMed ID: 28682648
10932  Shupeng WU, Jing WANG, Fang LI  Role of SNF5 in rheumatoid arthritis by up-regulation of p16 and inactivation of JNK 1 pathway  Turkish Journal of Biology  2017  41: 535-542 
7144  Miyashita T, Morimoto S, Fujishiro M, Hayakawa K, Suzuki S, Ikeda K, Miyazawa K, Morioka M, Takamori K, Ogawa H, Sekigawa I, Takasaki Y.  Inhibition of each module of connective tissue growth factor as a potential therapeutic target for rheumatoid arthritis.  Autoimmunity  2016  49:109-14  PubMed ID: 26584911
9866  Wu WJ, Jia WW, Liu XH, Pan LL, Zhang QY, Yang D, Shen XY, Liu L, Zhu YZ.  S-propargyl-cysteine attenuates inflammatory response in rheumatoid arthritis by modulating the Nrf2-ARE signaling pathway.  Redox Biol  2016  10:157-167  PubMed ID: 27744121
9879  Someya A, Ikegami T, Sakamoto K, Nagaoka I.  Glucosamine Downregulates the IL-1β-Induced Expression of Proinflammatory Cytokine Genes in Human Synovial MH7A Cells by O-GlcNAc Modification-Dependent and -Independent Mechanisms.  PLoS ONE  2016  11:e0165158  PubMed ID: 27776166
9157  Seiji Shibasaki, Miki Karasaki, Shunsuke Aburaya, Hironobu Morisaka, Yumiko Takeda, Wataru Aoki, Sachie Kitano, Masayasu Kitano, Mitsuyoshi Ueda, Hajime Sano, Tsuyoshi Iwasaki  A Comparative Proteomics Study of a Synovial Cell Line Stimulated With TNF-α  FEBS Open Bio  2016  6 (5), 418-24  PubMed ID: 27419047
7143  Imamura H, Yoshina S, Ikari K, Miyazawa K, Momohara S, Mitani S.  Impaired NFKBIE gene function decreases cellular uptake of methotrexate by down-regulating SLC19A1 expression in a human rheumatoid arthritis cell line.  Mod Rheumatol  2015  26:507-16  PubMed ID: 26587663
7188  Jia Q, Cheng W, Yue Y, Hu Y, Zhang J, Pan X, Xu Z, Zhang P.  Cucurbitacin E inhibits TNF-α-induced inflammatory cytokine production in human synoviocyte MH7A cells via suppression of PI3K/Akt/NF-κB pathways.  Int. Immunopharmacol.  2015  29:884-890  PubMed ID: 26453509
12290  Hideya Igarashi, Hiroyasu Hirano, Ayano Yahagi, Taro Saika, Katsuhiko Ishihara  Anti-apoptotic roles for the mutant p53R248Q through suppression of p53-regulated apoptosis-inducing protein 1 in the RA-derived fibroblast-like synoviocyte cell line MH7A  Clin Immunol  2014  150(1):12-21  PubMed ID: 24316591
10988  Geun-Hee Lee, Jiyoung Lee, Jae-Wook Lee, Whan Soo Choi, Eun-Yi Moon  B cell activating factor-dependent expression of vascular endothelial growth factor in MH7A human synoviocytes stimulated with tumor necrosis factor-α  Int Immunopharmacol  2013  17(1):142-7  PubMed ID: 23684916
7598  Fujishiro M, Nozawa K, Kawasaki M, Yamaguchi A, Iwabuchi K, Yanagida M, Suzuki F, Miyazawa K, Fukui H, Kaneko K, Ogawa H, Takamori K, Takasaki Y, Sekigawa I.  Regenerating gene (REG) 1 alpha promotes pannus progression in patients with rheumatoid arthritis.  Mod Rheumatol  2012  22:228-37  PubMed ID: 22203215
7631  Igarashi H, Yahagi A, Saika T, Hashimoto J, Tomita T, Yoshikawa H, Ishihara K.  A pro-inflammatory role for A20 and ABIN family proteins in human fibroblast-like synoviocytes in rheumatoid arthritis.  Immunol. Lett.  2012  141:246-53  PubMed ID: 22093807
7800  Tanaka Y, Matsumoto I, Iwanami K, Inoue A, Minami R, Umeda N, Kanamori A, Ochiai N, Miyazawa K, Sugihara M, Hayashi T, Goto D, Ito S, Sumida T.  Six-transmembrane epithelial antigen of prostate4 (STEAP4) is a tumor necrosis factor alpha-induced protein that regulates IL-6, IL-8, and cell proliferation in synovium from patients with rheumatoid arthritis.  Mod Rheumatol  2012  22:128-36  PubMed ID: 21633911
12717  Nakayama H, Yaguchi T, Yoshiya S, Nishizaki T.  Resveratrol induces apoptosis MH7A human rheumatoid arthritis synovial cells in a sirtuin 1-dependent manner  Rheumatol Int . 2012 Jan;32(1):151-7  2012  32(1):151-7  PubMed ID: 20697895
7779  Seong AR, Yoo JY, Choi K, Lee MH, Lee YH, Lee J, Jun W, Kim S, Yoon HG.  Delphinidin, a specific inhibitor of histone acetyltransferase, suppresses inflammatory signaling via prevention of NF-κB acetylation in fibroblast-like synoviocyte MH7A cells.  Biochem. Biophys. Res. Commun.  2011  410:581-6  PubMed ID: 21683061
7997  Bader RA, Silvers AL, Zhang N.  Polysialic acid-based micelles for encapsulation of hydrophobic drugs.  Biomacromolecules  2011  12:314-20  PubMed ID: 21218771
8289  Bader RA, Wagoner KL.  Modulation of the response of rheumatoid arthritis synovial fibroblasts to proinflammatory stimulants with cyclic tensile strain.  Cytokine  2010  51:35-41  PubMed ID: 20399680
8449  Nozawa K, Fujishiro M, Kawasaki M, Kaneko H, Iwabuchi K, Yanagida M, Suzuki F, Miyazawa K, Takasaki Y, Ogawa H, Takamori K, Sekigawa I.  Connective tissue growth factor promotes articular damage by increased osteoclastogenesis in patients with rheumatoid arthritis.  Arthritis Res. Ther.  2009  11:R174  PubMed ID: 19922639
10354  Masaji Morita 1, Yoshiko Banno, Taikoh Dohjima, Satoshi Nozawa, Kazunari Fushimi, De-Gang Fan, Takatoshi Ohno, Keiji Miyazawa, Naxin Liu, Katsuji Shimizu  Mu-calpain Is Involved in the Regulation of TNF-alpha-induced Matrix metalloproteinase-3 Release in a Rheumatoid Synovial Cell Line  Biochem Biophys Res Commun  2006  343(3):937-42  PubMed ID: 16574073

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