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Cell No. : Cell Name
RCB1509 : clone 64  update : 2022/04/04
CommentSubline of mouse C127 cell line. Inducible expression of influenza virus protein (PB1, NP, PA) is possible.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nakada, Susumu
Originator Nakada, Susumu
Year of deposit 1998
Original cell C127
Animal mouse < Mammals
Gender Female
Tissue mammary
Classification transformed
Recombinant recombinant
Exogene pBMSA, RNA polymerase (PB1, PA ), nucleoprotein
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_U341
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
2768  Nakagawa Y, Kimura N, Toyoda T, Mizumoto K, Ishihama A, Oda K, Nakada S  The RNA polymerase PB2 subunit is not required for replication of the influenza virus genome but is involved in capped mRNA synthesis.  J Virol  1995  69:728-33  PubMed ID: 7815536  
2767  Kimura N, Nishida M, Nagata K, Ishihama A, Oda K, Nakada S  Transcription of a recombinant influenza virus RNA in cells that can express the influenza virus RNA polymerase and nucleoprotein genes  Gen Virol  1992  73:1321-1328  PubMed ID: 1607855  

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