Cell No. : Cell Name
RCB1509 : clone 64
update : 2023/01/05
|
| Comment | Subline of mouse C127 cell line. Inducible expression of influenza virus protein (PB1, NP, PA) is possible. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nakada, Susumu
|
| Originator |
Nakada, Susumu
|
| Year of deposit |
1998
|
| Original cell |
C127
|
| Animal |
_mouse
< Mammals
|
| Gender |
Female
|
| Tissue |
mammary
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
pBMSA, RNA polymerase (PB1, PA ), nucleoprotein
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_U341
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Achoreplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
2768
Nakagawa Y, Kimura N, Toyoda T, Mizumoto K, Ishihama A, Oda K, Nakada S
The RNA polymerase PB2 subunit is not required for replication of the influenza virus genome but is involved in capped mRNA synthesis.
J Virol
1995
69:728-33
PubMed ID: 7815536
DOI: 10.1128/JVI.69.2.728-733.1995
|
|
2767
Kimura N, Nishida M, Nagata K, Ishihama A, Oda K, Nakada S
Transcription of a recombinant influenza virus RNA in cells that can express the influenza virus RNA polymerase and nucleoprotein genes
Gen Virol
1992
73:1321-1328
PubMed ID: 1607855
DOI: 10.1099/0022-1317-73-6-1321
|
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