Cell No. : Cell Name
RCB1506 : clone YP1N25
update : 2023/01/05
|
| Comment | Subline of mouse C127 cell line. Inducible expression of influenza virus protein (PB1, NP) is possible. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nakada, Susumu
|
| Originator |
Nakada, Susumu
|
| Year of deposit |
1998
|
| Original cell |
C127
|
| Animal |
_mouse
< Mammals
|
| Gender |
Female
|
| Tissue |
mammary
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
pBMSA, RNA polymerase (PB1), nucleoprotein
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_U349
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/2 weeks, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
2769
Nakagawa Y, Oda K, Nakada S
The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome.
J Virol
1996
70:6390-4
PubMed ID: 8709268
DOI: 10.1128/JVI.70.9.6390-6394.1996
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2766
Nakamura Y, Oda K, Nakada S
Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes.
J Biochem
1991
110:395-401
PubMed ID: 1663110
DOI: 10.1093/oxfordjournals.jbchem.a123592
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|
943
Lowy, D R, Rands E, Scolnick, E M
Helper-independent transformation by unintegrated Harvey sarcoma virus DNA.
J Virol
1978
26:291-8
PubMed ID: 26810
DOI: 10.1128/JVI.26.2.291-298.1978
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