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Cell No. : Cell Name
RCB1506 : clone YP1N25  update : 2022/04/04
CommentSubline of mouse C127 cell line. Inducible expression of influenza virus protein (PB1, NP) is possible.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nakada, Susumu
Originator Nakada, Susumu
Year of deposit 1998
Original cell C127
Animal mouse < Mammals
Gender Female
Tissue mammary
Classification transformed
Recombinant recombinant
Exogene pBMSA, RNA polymerase (PB1), nucleoprotein
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_U349
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
2769  Nakagawa Y, Oda K, Nakada S  The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome.  J Virol  1996  70:6390-4  PubMed ID: 8709268  
2766  Nakamura Y, Oda K, Nakada S  Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes.  J Biochem  1991  110:395-401  PubMed ID: 1663110  

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