Cell No. : Cell Name
RCB1502 : SHP2^(-) DT40 update : 2021/01/08 |
||||
Comment | SHP2 deficient DT40 cells | |||
Comment from the depositor | ||||
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
Remarks | ||||
Order Form | Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use. | |||
Basic information | Depositor | Kurosaki, Tomohiro | ||
Originator | Kurosaki, Tomohiro | |||
Year of deposit | 1998 | |||
Another name | M31-5 | |||
Original cell | DT40 | |||
Animal | avian < Birds | |||
Tissue | B cell | |||
Classification | mutant | |||
Recombinant | recombinant | Lifespan | infinite | |
Morphology | lymphocyte-like | |||
Medium | Medium List | Culture type | Suspension cells | |
Medium and additives | RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol | |||
Antibiotics | Free | Passage method | dilution | Culture information | Passage cell No | 1.5-2 x 10^(5) cells/ml |
Passage ratio | 1 : 20 split | |||
SC frequency | Subculture : every 2 days | |||
Temperature | 39.5 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | 90% FBS + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Animal PCR | OK | |||
Others | Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day. | |||
Reference information | Reference | 1 | ||
User's Publication | 1 | |||