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Cell No. : Cell Name
RCB1502 : SHP2^(-) DT40 update : 2021/01/08 |
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| Comment | SHP2 deficient DT40 cells | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use. | |||
| Basic information | Depositor | Kurosaki, Tomohiro | ||
| Originator | Kurosaki, Tomohiro | |||
| Year of deposit | 1998 | |||
| Another name | M31-5 | |||
| Original cell | DT40 | |||
| Animal | avian < Birds | |||
| Tissue | B cell | |||
| Classification | mutant | |||
| Recombinant | recombinant | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Medium and additives | RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage cell No | 1.5-2 x 10^(5) cells/ml |
| Passage ratio | 1 : 20 split | |||
| SC frequency | Subculture : every 2 days | |||
| Temperature | 39.5 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | 90% FBS + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| Animal PCR | OK | |||
| Others | Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day. | |||
| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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