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Cell No. : Cell Name
RCB1500 : Lyn^(-)/Syk^(-) DT40  update : 2024/11/13
CommentLyn and Sky deficient DT40 cells
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kurosaki, Tomohiro
Originator Kurosaki, Tomohiro
Year of deposit 1998
Another name S35-5
Original cell DT40
Animal _avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2H33
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10 5 cells/ml
Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Images
deposit info
lot info
Reference information Reference 2
User's Publication 1


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Reference
2282  Takata M, Kurosaki T.  A role for Bruton's tyrosine kinase in B cell antigen receptor-mediated activation of phospholipase C-gamma 2.  J Exp Med  1996  184(1):31-40  PubMed ID: 8691147   DOI: 10.1084/jem.184.1.31
2284  Takata M, Sabe H, Hata A, Inazu T, Homma Y, Nukada T, Yamamura H, Kurosaki T.  Tyrosine kinases Lyn and Syk regulate B cell receptor-coupled Ca2+ mobilization through distinct pathways.  EMBO J  1994  13(6):1341-9  PubMed ID: 8137818   DOI: 10.1002/j.1460-2075.1994.tb06387.x

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User's Publication
18613  de Gorter DJ, Vos JC, Pals ST, Spaargaren M.  The B cell antigen receptor controls AP-1 and NFAT activity through Ras-mediated activation of Ral  J Immunol  2007  178(3):1405-14  PubMed ID: 17237388   DOI: 10.4049/jimmunol.178.3.1405


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