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Cell No. : Cell Name
RCB1495 : FU-RPNT-1  update : 2022/04/04
CommentScarcely found primitive neuroectodermal tumor from a Japanese kidney. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Iwasaki, Hiroshi
Originator Takeuchi, Toshio
Year of deposit 1998
Another name SFT9421
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 22 years
Tissue kidney
Disease name primitive neuroectodermal tumo
Metastastatic ability Yes
Metastatsd tissue bone,lung, brain,etc
Classification cancer
Year of origin 1994
History Takeuchi, T., -> Iwasaki, H.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4837
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 43-48(50) : 43(2),45(8),46(10),47(29),48(1)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
2401  Takeuchi T, Iwasaki H, Ohjimi Y, Ohshima K, Kaneko Y, Ishiguro M, Hiratsuka Y, Sakamoto K, Kikuchi M  Renal primitive neuroectodermal tumor: a morphologic, cytogenetic, and molecular analysis with the establishment of two cultured cell lines.  Diagn Mol Pathol  1997  6:309-17  PubMed ID: 9559290  

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