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Cell No. : Cell Name
RCB1489 : RLC-27  update : 2023/01/05
CommentLiver-derived cell, tumorigenic in nude mice. Possible to culture in DMEM medium.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Originator Takaoka, T. & Katsuta, H.
Year of deposit 1998
Original cell RCB0072
Animal _rat < Mammals
Gender Male
Age at sampling 13 days
Tissue liver
Classification other
Year of origin 1974
Memo_1 RCB0072 : Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3862
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 5% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 1

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14  Sakai T, Kawakatsu H, Hirota N, Yokoyama T, Takoka T, Sakakura T, Saito M.  Tenascin expression in vitro and in vivo: comparison between epithelial and nonepithelial rat cell lines  Exp Cell Res  1993  206(2):244-54  PubMed ID: 7684694   DOI: 10.1006/excr.1993.1144

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User's Publication
3469  Uematsu, Fumiyuki, Takahashi, Masakazu, Yoshida, Midori, Igarashi, Maki, Nakae, Dai  Methylation of neutral endopeptidase 24.11 promoter in rat hepatocellular carcinoma.  Cancer Sci  2006  97:611-7  PubMed ID: 16827801   DOI: 10.1111/j.1349-7006.2006.00227.x

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