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Cell No. : Cell Name
RCB1486 : JTC-19  update : 2022/04/04
CommentRat lung fibroblast once said interferon-producing. Possible to culture in DMEM medium. See RCB0074 .
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Takaoka, Toshiko
Year of deposit 1998
Another name RLG-1
Animal rat < Mammals
Genus Rattus
Species norvegicus
Gender Female
Age at sampling 3 days
Tissue lung
Metastastatic ability No
Classification other
Year of origin 1964
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3632
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 5% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 0
User's Publication 0


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