Cell No. : Cell Name RCB1484 : RLC-18  update : 2022/04/04
Comment			Non tumorigenic in nude mice. Possible to culture in DMEM medium.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ohno, Tadao
		Originator	Yasumoto,Shigeru et al.
		Year of deposit	1998
		Original cell	RCB0070
		Animal	_rat < Mammals
		Age at sampling	embryo
		Tissue	liver
		Classification	other
		Memo_1	RCB0070 : Mycoplasma was eliminated with MC-210.
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_2178
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (low glucose) + 5% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
Reference information		Reference	1
		User's Publication	2

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Reference

993  Takaoka T, Yasumoto S, Katsuta H.  A simple method for the cultivation of rat liver cells.  Jpn J Exp Med  1975  45(5):317-26  PubMed ID: 1223332

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User's Publication

16322  Munetsuna E, Yamada H, Yamazaki M, Ando Y, Mizuno G, Hattori Y, Kageyama I, Teshigawara A, Nouchi Y, Ishikawa H, Fujii R, Ohta Y, Suzuki K, Shimono Y, Ohashi K, Hashimoto S.  Maternal fructose intake predisposes rat offspring to metabolic disorders via abnormal hepatic programming  FASEB J  2021  35(12):e22030  PubMed ID: 34748238   DOI: 10.1096/fj.202101276R

8996  Shinohara M, Kawashima S, Yamashita T, Takaya T, Toh R, Ishida T, Ueyama T, Inoue N, Hirata K, Yokoyama M.  Xenogenic smooth muscle cell immunization reduces neointimal formation in balloon-injured rabbit carotid arteries.  Cardiovasc. Res.  2005  68:249-58  PubMed ID: 16039635   DOI: 10.1016/j.cardiores.2005.06.016