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Cell No. : Cell Name
RCB1484 : RLC-18  update : 2022/04/04
CommentNon tumorigenic in nude mice. Possible to culture in DMEM medium.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Yasumoto,Shigeru et al.
Year of deposit 1998
Original cell RCB0070
Animal rat < Mammals
Age at sampling embryo
Tissue liver
Classification other
Memo_1 RCB0070 : Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_2178
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 5% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 2


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Reference
993  Takaoka T, Yasumoto S, Katsuta H.  A simple method for the cultivation of rat liver cells.  Jpn J Exp Med  1975  45(5):317-26  PubMed ID: 1223332

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User's Publication
16322  Munetsuna E, Yamada H, Yamazaki M, Ando Y, Mizuno G, Hattori Y, Kageyama I, Teshigawara A, Nouchi Y, Ishikawa H, Fujii R, Ohta Y, Suzuki K, Shimono Y, Ohashi K, Hashimoto S.  Maternal fructose intake predisposes rat offspring to metabolic disorders via abnormal hepatic programming  FASEB J  2021  35(12):e22030  PubMed ID: 34748238
8996  Shinohara M, Kawashima S, Yamashita T, Takaya T, Toh R, Ishida T, Ueyama T, Inoue N, Hirata K, Yokoyama M.  Xenogenic smooth muscle cell immunization reduces neointimal formation in balloon-injured rabbit carotid arteries.  Cardiovasc. Res.  2005  68:249-58  PubMed ID: 16039635



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