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Cell No. : Cell Name
RCB1479 : JTC-27  update : 2025/02/26
CommentAscites hepatoma. Possible to culture in DMEM medium.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Huh, Namho et al
Year of deposit 1998
Animal _rat < Mammals
Tissue ascites hepatoma
Classification cancer
Year of origin 1971
Memo_1 RCB0065 : Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_2085
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 5% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
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lot info
Reference information Reference 2
User's Publication 0


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Reference
3666  高岡 聰子,秋元 一三  組織培養におけるエラスターゼの作用  組織培養研究  1984  3:31-32   
989  Huh N, Takaoka T, Katsuta H  Tissue culture of rat ascites hepatoma AH-601 cells.  Jpn J Exp Med  1972  42:249-62  PubMed ID: 4340679  

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