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Cell No. : Cell Name
RCB1477 : CHO-RD  update : 2022/11/17
CommentSubline of CHO cells. Derived from CHO-K1 cell line. Serum-free medium adapted. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Inazu, Mizuho
Originator Fujino, Y. & Watabe, H.
Year of deposit 1998
Original cell CHO-K1
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification other
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4669
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives (RPMI1640 : DMEM = 2 : 1) + 5μg/ml Insulin + 10μg/ml Transferrin + 0.1% Pluronic F-68 + 1.53nl/ml Ethanolamine + 0.0043μg/ml Sodium Selenite
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
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Reference information Reference 0
User's Publication 0


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