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Cell No. : Cell Name
RCB1474 : RLC-16  update : 2022/11/17
CommentEpithelial cell from rat liver. Possible to culture in DMEM medium.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Originator Takaoka, Toshiko
Year of deposit 1998
Animal rat < Mammals
Genus Rattus
Species norvegicus
Gender Female
Tissue liver
Classification other
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_3861
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 5% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 4

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993  Takaoka T, Yasumoto S, Katsuta H.  A simple method for the cultivation of rat liver cells.  Jpn J Exp Med  1975  45(5):317-26  PubMed ID: 1223332

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User's Publication
13852  Nakagawa H, Komori M, Nishimura K.  Carbon tetrachloride suppresses ER-Golgi transport by inhibiting COPII vesicle formation on the ER membrane in the RLC-16 hepatocyte cell line  Cell Biol Int  2021  45(3):633-641  PubMed ID: 33247607
4340  Nagatome M, Kondo Y, Kadowaki D, Saishyo Y, Irikura M, Irie T, Ishitsuka Y.  Ethyl pyruvate attenuates acetaminophen-induced liver injury and prevents cellular injury induced by N-acetyl-p-benzoquinone imine.  Heliyon  2018    PubMed ID: 29560444
15958  Tomishima Y, Ishitsuka Y, Matsunaga N, Nagatome M, Furusho H, Irikura M, Ohdo S, Irie T.  Ozagrel hydrochloride, a selective thromboxane A₂ synthase inhibitor, alleviates liver injury induced by acetaminophen overdose in mice  BMC Gastroenterol  2013  13:21  PubMed ID: 23363429
3469  Uematsu, Fumiyuki, Takahashi, Masakazu, Yoshida, Midori, Igarashi, Maki, Nakae, Dai  Methylation of neutral endopeptidase 24.11 promoter in rat hepatocellular carcinoma.  Cancer Sci  2006  97:611-7  PubMed ID: 16827801

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