Cell No. : Cell Name
RCB1474 : RLC-16
update : 2022/11/17
|
| Comment | Epithelial cell from rat liver. Possible to culture in DMEM medium. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Ohno, Tadao
|
| Originator |
Takaoka, Toshiko
|
| Year of deposit |
1998
|
| Animal |
_rat
< Mammals
|
| Genus |
Rattus
|
| Species |
norvegicus
|
| Gender |
Female
|
| Tissue |
liver
|
| Classification |
other
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_3861
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 5% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
4
|
| User's Publication |
|
13852
Nakagawa H, Komori M, Nishimura K.
Carbon tetrachloride suppresses ER-Golgi transport by inhibiting COPII vesicle formation on the ER membrane in the RLC-16 hepatocyte cell line
Cell Biol Int
2021
45(3):633-641
PubMed ID: 33247607
DOI: 10.1002/cbin.11510
|
|
4340
Nagatome M, Kondo Y, Kadowaki D, Saishyo Y, Irikura M, Irie T, Ishitsuka Y.
Ethyl pyruvate attenuates acetaminophen-induced liver injury and prevents cellular injury induced by N-acetyl-p-benzoquinone imine.
Heliyon
2018
PubMed ID: 29560444
DOI: 10.1016/j.heliyon.2018.e00521
|
|
15958
Tomishima Y, Ishitsuka Y, Matsunaga N, Nagatome M, Furusho H, Irikura M, Ohdo S, Irie T.
Ozagrel hydrochloride, a selective thromboxane A₂ synthase inhibitor, alleviates liver injury induced by acetaminophen overdose in mice
BMC Gastroenterol
2013
13:21
PubMed ID: 23363429
DOI: 10.1186/1471-230X-13-21
|
|
3469
Uematsu, Fumiyuki, Takahashi, Masakazu, Yoshida, Midori, Igarashi, Maki, Nakae, Dai
Methylation of neutral endopeptidase 24.11 promoter in rat hepatocellular carcinoma.
Cancer Sci
2006
97:611-7
PubMed ID: 16827801
DOI: 10.1111/j.1349-7006.2006.00227.x
|
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