Cell No. : Cell Name RCB1470 : Syk^(-) DT40  update : 2024/11/13
Comment			Syk deficient DT40 cells
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Kurosaki, Tomohiro
		Originator	Kurosaki, Tomohiro
		Year of deposit	1998
		Another name	S1-10
		Original cell	DT40
		Animal	_avian < Birds
		Tissue	B cell
		Classification	mutant
		Recombinant	recombinant
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_1T43
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage cell No		1.5-2 x 10 5 cells/ml
		Passage ratio		1 : 20 split
		SC frequency		Subculture : every 2 days
		Temperature		39.5 ℃
		CO2 concentration		5 %
		Freeze medium		90% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Others		Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	3

Top

Reference

2284  Takata M, Sabe H, Hata A, Inazu T, Homma Y, Nukada T, Yamamura H, Kurosaki T.  Tyrosine kinases Lyn and Syk regulate B cell receptor-coupled Ca2+ mobilization through distinct pathways.  EMBO J  1994  13(6):1341-9  PubMed ID: 8137818   DOI: 10.1002/j.1460-2075.1994.tb06387.x

Top

User's Publication

19601  Singaram I, Sharma A, Pant S, Lihan M, Park MJ, Pergande M, Buwaneka P, Hu Y, Mahmud N, Kim YM, Cologna S, Gevorgyan V, Khan I, Tajkhorshid E, Cho W.  Targeting lipid-protein interaction to treat Syk-mediated acute myeloid leukemia  Nat Chem Biol  2023  19(2):239-250  PubMed ID: 36229686   DOI: 10.1038/s41589-022-01150-z

8442  Liu Z, Mai A, Sun J.  Lysine acetylation regulates Bruton's tyrosine kinase in B cell activation.  J. Immunol.  2010  184:244-54  PubMed ID: 19949111   DOI: 10.4049/jimmunol.0902324

17351  Blois JT, Mataraza JM, Mecklenbraüker I, Tarakhovsky A, Chiles TC.  B cell receptor-induced cAMP-response element-binding protein activation in B lymphocytes requires novel protein kinase Cdelta  J Biol Chem  2004  279(29):30123-32  PubMed ID: 15138267   DOI: 10.1074/jbc.M402793200