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Cell No. : Cell Name
RCB1470 : Syk^(-) DT40  update : 2024/01/17
CommentSyk deficient DT40 cells
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kurosaki, Tomohiro
Originator Kurosaki, Tomohiro
Year of deposit 1998
Another name S1-10
Original cell DT40
Animal _avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1T43
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10 5 cells/ml
Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
deposit info
lot info
Reference information Reference 1
User's Publication 3

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2284  Takata M, Sabe H, Hata A, Inazu T, Homma Y, Nukada T, Yamamura H, Kurosaki T.  Tyrosine kinases Lyn and Syk regulate B cell receptor-coupled Ca2+ mobilization through distinct pathways.  EMBO J  1994  13(6):1341-9  PubMed ID: 8137818   DOI: 10.1002/j.1460-2075.1994.tb06387.x

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User's Publication
19601  Singaram I, Sharma A, Pant S, Lihan M, Park MJ, Pergande M, Buwaneka P, Hu Y, Mahmud N, Kim YM, Cologna S, Gevorgyan V, Khan I, Tajkhorshid E, Cho W.  Targeting lipid-protein interaction to treat Syk-mediated acute myeloid leukemia  Nat Chem Biol  2023  19(2):239-250  PubMed ID: 36229686   DOI: 10.1038/s41589-022-01150-z
8442  Liu Z, Mai A, Sun J.  Lysine acetylation regulates Bruton's tyrosine kinase in B cell activation.  J. Immunol.  2010  184:244-54  PubMed ID: 19949111   DOI: 10.4049/jimmunol.0902324
17351  Blois JT, Mataraza JM, Mecklenbraüker I, Tarakhovsky A, Chiles TC.  B cell receptor-induced cAMP-response element-binding protein activation in B lymphocytes requires novel protein kinase Cdelta  J Biol Chem  2004  279(29):30123-32  PubMed ID: 15138267   DOI: 10.1074/jbc.M402793200

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