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Cell No. : Cell Name
RCB1469 : PLC-γ2^(-) DT40  update : 2024/11/13
CommentPLC-γ2 deficient DT40 cells
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kurosaki, Tomohiro
Originator Kurosaki, Tomohiro
Year of deposit 1998
Another name P10-14
Original cell DT40
Animal _avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1T47
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10 5 cells/ml
Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Images
deposit info
lot info
Reference information Reference 3
User's Publication 4


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Reference
2337  Hashimoto A, Okada H, Jiang A, Kurosaki M, Greenberg S, Clark EA, Kurosaki T.  Involvement of guanosine triphosphatases and phospholipase C-gamma2 in extracellular signal-regulated kinase, c-Jun NH2-terminal kinase, and p38 mitogen-activated protein kinase activation by the B cell antigen receptor.  J Exp Med  1998  188(7):1287-95  PubMed ID: 9763608   DOI: 10.1084/jem.188.7.1287
2281  Sugawara H, Kurosaki M, Takata M, Kurosaki T.  Genetic evidence for involvement of type 1, type 2 and type 3 inositol 1,4,5-trisphosphate receptors in signal transduction through the B-cell antigen receptor.  EMBO J  1997  16(11):3078-88  PubMed ID: 9214625   DOI: 10.1093/emboj/16.11.3078
2283  Takata M, Homma Y, Kurosaki T.  Requirement of phospholipase C-gamma 2 activation in surface immunoglobulin M-induced B cell apoptosis.  J Exp Med  1995  182(4):907-14  PubMed ID: 7561693   DOI: 10.1084/jem.182.4.907

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User's Publication
7115  Zaldua N, Llavero F, Artaso A, Galvez P, Lacerda HM, Parada LA, Zugaza JL.  Rac1/p21-activated kinase pathway controls retinoblastoma protein phosphorylation and E2F transcription factor activation in B lymphocytes.  FEBS J.  2016  283:647-61  PubMed ID: 26663827   DOI: 10.1111/febs.13617
15039  Walliser C, Tron K, Clauss K, Gutman O, Kobitski AY, Retlich M, Schade A, Röcker C, Henis YI, Nienhaus GU, Gierschik P.  Rac-mediated Stimulation of Phospholipase Cγ2 Amplifies B Cell Receptor-induced Calcium Signaling  J Biol Chem  2015  290(28):17056-72  PubMed ID: 25903139   DOI: 10.1074/jbc.M115.645739
18613  de Gorter DJ, Vos JC, Pals ST, Spaargaren M.  The B cell antigen receptor controls AP-1 and NFAT activity through Ras-mediated activation of Ral  J Immunol  2007  178(3):1405-14  PubMed ID: 17237388   DOI: 10.4049/jimmunol.178.3.1405
17351  Blois JT, Mataraza JM, Mecklenbraüker I, Tarakhovsky A, Chiles TC.  B cell receptor-induced cAMP-response element-binding protein activation in B lymphocytes requires novel protein kinase Cdelta  J Biol Chem  2004  279(29):30123-32  PubMed ID: 15138267   DOI: 10.1074/jbc.M402793200


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