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Cell No. : Cell Name
RCB1467 : IP3R^(-) DT40  update : 2024/01/17
CommentIP3R(type1,type2,type3) deficient DT40 cells
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kurosaki, Tomohiro
Originator Kurosaki, Tomohiro
Year of deposit 1998
Another name R23-11
Original cell DT40
Animal _avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4634
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10 5 cells/ml
Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
deposit info
lot info
Reference information Reference 2
User's Publication 9

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2337  Hashimoto A, Okada H, Jiang A, Kurosaki M, Greenberg S, Clark EA, Kurosaki T.  Involvement of guanosine triphosphatases and phospholipase C-gamma2 in extracellular signal-regulated kinase, c-Jun NH2-terminal kinase, and p38 mitogen-activated protein kinase activation by the B cell antigen receptor.  J Exp Med  1998  188(7):1287-95  PubMed ID: 9763608   DOI: 10.1084/jem.188.7.1287
2281  Sugawara H, Kurosaki M, Takata M, Kurosaki T.  Genetic evidence for involvement of type 1, type 2 and type 3 inositol 1,4,5-trisphosphate receptors in signal transduction through the B-cell antigen receptor.  EMBO J  1997  16(11):3078-88  PubMed ID: 9214625   DOI: 10.1093/emboj/16.11.3078

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User's Publication
16819  Horia Vais, Min Wang, Karthik Mallilankaraman, Riley Payne, Chris McKennan, Jeffrey T Lock, Lynn A Spruce, Carly Fiest, Matthew Yan-lok Chan, Ian Parker, Steven H Seeholzer, J Kevin Foskett, Don-On Daniel Mak  ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein  eLife  2020  9  PubMed ID: 32420875   DOI: 10.7554/elife.53531
14706  Ren G, Zhou Y, Liang G, Yang B, Yang M, King A, Wei H.  General Anesthetics Regulate Autophagy via Modulating the Inositol 1,4,5-Trisphosphate Receptor: Implications for Dual Effects of Cytoprotection and Cytotoxicity  Sci Rep  2017  7(1):12378  PubMed ID: 28959036   DOI: 10.1038/s41598-017-11607-0
15634  Rahman T, Taylor CW.  Nuclear patch-clamp recording from inositol 1,4,5-trisphosphate receptors  Methods Cell Biol  2010  99:199-224  PubMed ID: 21035688   DOI: 10.1016/B978-0-12-374841-6.00008-6
8598  Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N.  Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.  PLoS Genet.  2009  5:e1000461  PubMed ID: 19390601   DOI: 10.1371/journal.pgen.1000461
9376  Wedel BJ, Vazquez G, McKay RR, St J Bird G, Putney JW Jr.  A calmodulin/inositol 1,4,5-trisphosphate (IP3) receptor-binding region targets TRPC3 to the plasma membrane in a calmodulin/IP3 receptor-independent process.  J. Biol. Chem.  2003  278:25758-65  PubMed ID: 12730194   DOI: 10.1074/jbc.M303890200
9379  Vazquez G, Wedel BJ, Trebak M, St John Bird G, Putney JW Jr.  Expression level of the canonical transient receptor potential 3 (TRPC3) channel determines its mechanism of activation.  J. Biol. Chem.  2003  278:21649-54  PubMed ID: 12686562   DOI: 10.1074/jbc.M302162200
9416  Vazquez G, Wedel BJ, Bird GS, Joseph SK, Putney JW.  An inositol 1,4,5-trisphosphate receptor-dependent cation entry pathway in DT40 B lymphocytes.  EMBO J.  2002  21:4531-8  PubMed ID: 12198155   DOI: 10.1093/emboj/cdf467
9450  Vazquez G, Lievremont JP, St J Bird G, Putney JW Jr.  Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes.  Proc. Natl. Acad. Sci. U.S.A.  2001  98:11777-82  PubMed ID: 11553786   DOI: 10.1073/pnas.201238198
9452  Broad LM, Braun FJ, Lievremont JP, Bird GS, Kurosaki T, Putney JW Jr.  Role of the phospholipase C-inositol 1,4,5-trisphosphate pathway in calcium release-activated calcium current and capacitative calcium entry.  J. Biol. Chem.  2001  276:15945-52  PubMed ID: 11278938   DOI: 10.1074/jbc.M011571200

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